Obviously the research community is not satisfied with 2D-imaging anymore. To have more information about depth of the signal BERTHOLD TECHNOLOGIES has developed an orthogonal 3D-Imaging option. 
This option allows imaging of mice from top, left and right side. The sample is moved on the tray without changing the position. To obtain images from left or right side with the same magnification the camera position has to be lowered according to the same working distance.

With indiGo software the researcher is able to calculate roughly the volume of the illuminated spot, but it is not possible to do any reconstruction due to the inverse problem.

To solve the inverse problem in bioluminescence, another set of data with another technology has to be aquired. This technology may be X-ray-CT or MRI combined with optical imaging. BERTHOLD TECHNOLOGIES has a strong focus on such multimodal imaging technologies, combined with the adequate multimodal software packages.

Correlation between luminescent signal and tumour volume during exponential proliferation. Luciferase activity and manually measured xenograft volume were plotted from five different mice that gave 19 separate measurements (r2=0,8888). Tumour volume (mm3) was estimated by the formula d1xd2xd3/2 (d1=tumour length, d2=width, d3=height).

Some techniques for generating and/or detecting light in biological subjects are patented and may require licences from third parties. Users are advised to independently determine for themselves wheter their activities infringe any valid patent.