Orion II LB 965 Microplate Luminometer Brochure
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The Orion II LB 965 meets all currently known requirements for a. The Orion II supports all basic luminescence technologies including:
The outstanding sensitivity of Orion II is the result of the unique design, advanced engineering, and high-quality workmanship. All functions of the Orion II are seamlessly integrated to render superior, user-friendly performance.
The detector is operated in photon counting mode which guarantees the lowest signal background for an excellent signal to noise ratio with the highest linearity. The built-in safety circuit protects the detector from potential damage resulting from accidental exposure to high levels of light. A warning is also produced should a sample ever exceed measurement range.
The Orion II is more than just a basic, for assays that require shaking, three mixing modes are available: linear, orbital and cross shaking. Flexible speed and amplitude settings allow easy adaptation to any assay. Orion II offers two different types of sample incubation: top and bottom microplate incubation ensures exceptional temperature stability in the sample chamber for temperature control up to 50°C. For applications that require up to 42°C incubation only, the optional bottom heating function is recommended.
Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples? Then you´re potentially affected by a phenomenon called, that can produce wrong results and wrong conclusions. The special design of the Orion IIalmost eliminates this problem. With a crosstalk of 3 x 10-5, you never have to worry about the position of your high-intensity samples in your plate.
The combination of reagent delivery options is virtually unlimited. Up to four automatic reagent injectors are available with injection volumes of 10 to 150 μL each. For increased throughput, up to 2 injectors can be installed in measurement position for simultaneous injection. The optimized reagent holders accommodate any shape and any size bottle in a position that allows the complete use of valuable reagent.
Thanks to a built-in waste pump you will not need to worry about loading microplates, waste containers or removal of injector tips for washing and filling the reagent lines. The built-in waste pump takes care of the safe removal of all excess liquids.
The Orion II system has the capability to run samples and process measurements in the most time efficient manner. The proprietary Double Injection Design (DID) allows parallel processing of samples saving valuable assay time. DID has been applied to both 96 well and 384 well configurations.
Low-noise photomultiplier tube in single photon counting mode
|Measurement modes||Endpoint, Kinetics, Repeated, Batch|
|Spectral range luminescence||300-600 nm|
|Injectors||Up to 4 syringe injectors|
|Injector volume||10-150 µL in 1 µL steps|
|Microplate formats||96- and 384-well|
|Shaking||Linear, orbital, cross shaking, with selectable speed|
|Temperature control||From ambient +5° to 42° C or to 50° C (optional)|
|Luminescence sensitivity||<10 amol ATP/well (96-well plate)|
|Crosstalk||3 x 10-5|
|Dynamic range||6 orders of magnitude|
|Interface||Windows software, connected to computer by USB|
|Operating system||Windows 7 or better|
|Available software packages|| |
|Power Supply||110-240 V, 50/60 Hz, 30 VA (maximum 70 VA with 4 injectors and heating)|
|Temperature tolerance||Storage: 0-40° C|
Operation: 15-30° C
Dimensions (W x D x H)
|385 x 410 x 255 mm|
DLR Assay (Promega) with the Orion II Dual Luciferase Reporter Assay (Promega) with the Orion II Microplate Luminometer
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Listeria and Salmonella Detection using Crocodile and Orion II 24 h Listeria and Salmonella Detection in food matrices using rRNA based LUMIprobe 24 Method with the Crocodile Assay miniWorkstation and the Orion II Microplate Luminometer
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Intracellular Ca2+ with Clonetics™ Primary Sensors and the Orion II Monitoring Intracellular Ca2+ Fluxes with Clonetics™ Primary Sensors using the Orion II Microplate Luminometer
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ABEL® Cell Activation Assay with the Orion II ABEL® Cell Activation Assay for Whole Blood or Isolated Cells with Pholasin® and Adjuvant-K™ with the Orion II Microplate Luminometer
PDF | 255.9 KB
ViaLight™ Assay with the Orion II ViaLight™ Cell proliferation and Cytotoxicity BioAssay Kit (Lonza) with the Orion II Microplate Luminometer
PDF | 67.5 KB
ToxiLight™ Cytotoxicity Assay with the Orion II ToxiLight™ Non-Destructive Cytotoxicity Bio Assay Kit (Lonza) with the Orion II Microplate Luminometer
PDF | 54.8 KB
MycoAlert® Assay with the Orion II MycoAlert® Mycoplasma Detection Assay (Lonza) with the Orion II Microplate Luminometer
PDF | 49.4 KB
Abel® Antioxidant Assay with the Orion II Abel® Antioxidant Assay with Superoxide with the Orion II Microplate Luminometer
PDF | 63.4 KB
ATP measurements with the Orion II Comparison of standard curves for ATP with the Orion II Microplate Luminometer
PDF | 200.4 KB
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Berthold Technologies GmbH & Co.KG
Calmbacher Str. 22
75323 Bad Wildbad
Tel.: (+49) 7081 177 0
Fax: (+49) 7081 177 100