AlphaScreen

AlphaScreen® relies on hydrogel coated Donor and Acceptor beads providing functional groups for conjugation to biomolecules.

The beads come in close proximity when the biomolecules interact by molecular binding, e.g. antibody-antigen or receptor-ligand. Laser excitation at 680 nm of a photosensitiser present on the Donor bead results in the production of singlet oxygen. The singlet oxygen migrates to react with chemiluminescers on the Acceptor bead. The chemiluminescers then activates fluorophores emitting light at 520 - 620 nm. This reaction cascade results in a huge amplification of the signal.

AlphaScreen® has proven to be a reliable and sensitive method in detection assays for secondary messengers like cyclic AMP or IP3 activity. In addition it is a homogenous method well suited for miniaturisation and the use in drug discovery screening.

AlphaScreen is a registered trademark of PerkinElmer.

 

Suitable Berthold instruments:

Mithras LB 940 Multimode Microplate Reader

Mithras multilabel reader frontview
modular and reliable multimode, multilabel, multi-detection microplate reader with outstanding performance in all technologies

Mithras² LB 943 Monochromator Multimode Reader

Mithras² Monochromator Multimode Reader
multimode, multilabel, multidetection, multitechnology microplate reader with monochromators and filters