Centro LB 960 Microplate Luminometer

The Universal Microplate Luminometer

The Centro LB 960 meets all currently known requirements for a versatile, robust and sensitive microplate luminometer. The reader supports all basic luminescence technologies including

  • Flash-type Luminescence
  • Glow-type Luminescence
  • Cellular Luminescence
  • Bio-Luminescence
  • Chemi-Luminescence

The selected detector and the proprietary design of the optical system guarantee lowest background and negligible crosstalk.

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Features

Built-in Reagent Injectors

Up to 3 reagent injectors can be set up to dispense multiple activators or detection reagents at any time and with user-adjustable volumes during the measurement.
Berthold Technologies´ injectors are based on the proprietary JET Injection technology. Superior accuracy and precision is combined with excellent mixing performance. Variable injections speeds and "friendly" material enable even injection of live cells (e.g. in Aequorin based GPCR screening).
One of the injector tips is located in reading position enabling the measurement of very fast labels, e.g. Acridinium esters, and to inject activators into a running kinetic.

Waste Pump

Excess fluids from priming and washing procedures are safely discarded via an automatic waste pump.

Temperature Control

A temperature controlled microplate compartment ensures stable conditions whenever temperature sensitive enzymes or cells are in use. Unique to the Centro a sandwich-type heating plate system guarantees optimised precision and accuracy.

Small Footprint

The design of the LB 960 Centro requires minimum bench space. All the important features are built in allowing maximum use of available bench space. The reagent injectors are housed within the small footprint, providing the advantage of short tubing and thus a low dead volume.

Specifications
Detection Deviceslow-noise photomultiplier tube operated in single photon counting mode, spectral range 340 to 630 nm
Sample/Plate Formats96 and 384 well plates
filter membranes
Temperature Controlup to 42 °C
Shaking3 modes, 3 velocities
Injection   up to 3 JET injectors
variable volume 10 – 100 µL
variable speed
Kinetics Operationminimum resolution 0.05 sec
maximum duration up to 7 days
Automation 
Performance 
Luminescence   <5 amol ATP per well
<2 zmol Firefly luciferase per well
Crosstalk1 x 10-6
Dynamic range>6 decades (PMT)
Injectorsaccuracy better 98 % over entire volume range
precision better 98 % over entire volume range
Miscellaneous 
Dimensions390 x 450 x 350 mm (w x d x h)
incl. injectors
OSWin XP, Win Vista, Win 7
RegulationsCE

 

Accessories

Mikrowin software

This PC software combines operation and definition of instrument settings as well as data reduction and evaluation. The wells for measurement and injection can be selected independently. Various operations can be picked and placed in any order to accommodate for all different requirements of the assays to be performed.
Kinetic data reduction and graphical display of the respective curves help the user to judge the results.
Any type of ratio calculation, e.g. DLR®, or other mathematical formulas can be linked to every well individually.
Standard curve fitting and calculation of unknown samples is available for those users looking for quantification of their results (Advanced I and II).
All data can be exported in EXCEL® or ASCII formats. Instrument settings, user
signatures and file information are stored with the results.
The password system and the audit trail function of the Advanced I and II versions are important tools for data security.

Robot Integration

Specially designed for but not restricted to the use in HTS departments of drug discovery companies the robot access module allows for uncomplicated integration of the Mithras into any type of lab automation system. Software and hardware easily integrate into existing robotic or liquid handling systems.

384 Well Reading

The instrument can be equipped with a software-driven device to automatically change between matching apertures for 96 and 384 well plates. Using matching apertures is the only safe and reliable way to get optimised efficiency while maintaining minimised crosstalk.

Adapters

For filter membranes a special adapter is available to enable reading of luminescent dot blots.

Test Plates & QC Services

For continuous verification of the instrument performance test plates or test kits can be applied by the user and Performance Qualification services done by experienced Berthold Technologies personnel are available.
 

Cleanit Daily Injector Cleaning Solution

For proper maintenance of the injection system the Cleanit Daily solution is recommended for regular cleaning. Daily cleaning ensures that accuracy and precision as well as a long life time of the injectors will be maintained.

Applications

For Applications of Centro microplate luminometer see here


For detailed application information and selection of the best suited instrument please have a look to the Centro Selection Guide.

Download
Brochure
Format Size
Bioanalytic Instruments - Product Portfolio Brief overview scope of supply Bioanalytic (June 2014) de, en PDF 693.31 KB
Centro / Centro XS3 LB 960 Brochure 2012 de, en PDF 1.43 MB
Order Information Consumables & QC Test Devices Version Jan. 2017 de, en PDF 153.63 KB
Press & Media
Format Size
2012-04 (April 2012) Centro microplate luminometer NL Centro isuue Berthold Technologies Newsletter April 2012 de, en PDF 169.99 KB
2013-03 (March 2013) Sensitivity & Centro XS³ NL The Truth about Sensitivity & Centro XS³ Issue - Berthold Technologies Newsletter March 2013 de, en PDF 86.86 KB

News about this product

This educational JoVE video explains in vitro transcription and capping of mRNA and subsequent transfection into HeLa cells and final measurement of luciferase activity in a Centro microplate luminometer

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10.02.2015

PPIs represent a major target in drug discovery. Estimations reach up to half a Million of interactions in humans.

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18.11.2013

Wir gratulieren Herrn Professor Dr. Shinya Yamanaka recht herzlich  zu seinem diesjährigen Nobelpreis in Medizin: "for the discovery that mature cells can be reprogrammed to become pluripotent"

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12.10.2012

We congratulate Professor Dr. Shinya Yamanaka for winning this year's Nobel Prize Award in Medicine: "for the discovery that mature cells can be reprogrammed to become pluripotent".

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12.10.2012