Centro XS³ LB 960 Microplate Luminometer

The High Sensitivity Microplate Luminometer


The CentroXS³ LB 960 meets all currently known requirements for a versatile, robust and sensitive microplate luminometer. The reader supports all basic luminescence technologies including

  • Flash-type Luminescence, e.g reporter gene assays
  • Glow-type Luminescence

The selected detector and the proprietary design of the optical system guarantee lowest background and negligible crosstalk. It is the instrument of choice for reporter gene assays and ATP determination when highest sensitivity is required.

Tabs

Features

Built-in Reagent Injectors

Up to 3 reagent injectors can be set up to dispense multiple activators or detection reagents at any time and with user-adjustable volumes during the measurement.
Berthold Technologies´ injectors are based on the proprietary JET Injection technology. Superior accuracy and precision is combined with excellent mixing performance. Variable injections speeds and "friendly" material enable even injection of live cells (e.g. in Aequorin based GPCR screening).

Waste Pump

Excess fluids from priming and washing procedures are safely discarded via an automatic waste pump.

Small Footprint

The design of the LB 960 Centro requires minimum bench space. All the important features are built in allowing maximum use of available bench space. The reagent injectors are housed within the small footprint, providing the advantage of short tubing and thus a low dead volume.

Specifications
Detection Deviceslow-noise photomultiplier tube operated in single photon counting mode, spectral range 340 to 630 nm
Sample/Plate Formats96 and 384 well plates
filter membranes
Shaking3 modes, 3 velocities
Injectionup to 3 JET injectors
variable volume 10 – 100 µL
variable speed
Kinetics Operationminimum resolution 0.05 sec
maximum duration up to 7 days
Automationrobot integration
Performance 
Luminescence   <1 amol ATP per well
<1 zmol Firefly luciferase per well
Crosstalk   1 x 10-6
Dynamic range>6 decades (PMT)
Injectorsaccuracy better 98 % over entire volume range
precision better 98 % over entire volume range
Miscellaneous 
Dimensions   330 x 430 x 295 mm (w x d x h)
incl. injectors
OSWin XP, Win Vista, Win 7
RegulationsCE

 

Accessories

Mikrowin software

This PC software combines operation and definition of instrument settings as well as data reduction and evaluation. The wells for measurement and injection can be selected independently. Various operations can be picked and placed in any order to accommodate for all different requirements of the assays to be performed.
Kinetic data reduction and graphical display of the respective curves help the user to judge the results.
Any type of ratio calculation, e.g. DLR®, or other mathematical formulas can be linked to every well individually.
Standard curve fitting and calculation of unknown samples is available for those users looking for quantification of their results (Advanced I and II).
All data can be exported in EXCEL® or ASCII formats. Instrument settings, user
signatures and file information are stored with the results.
The password system and the audit trail function of the Advanced I and II versions are important tools for data security.

Robot Integration

Specially designed for but not restricted to the use in HTS departments of drug discovery companies the robot access module allows for uncomplicated integration of the Mithras into any type of lab automation system. Software and hardware easily integrate into existing robotic or liquid handling systems.

384 Well Reading

The instrument is equipped with exchangeable apertures for 96 and 384 well plates. Using matching apertures is the only safe and reliable way to get optimised efficiency while maintaining minimised crosstalk.

Adapters

For filter membranes a special adapter is available to enable reading of luminescent dot blots.

Test Plates & QC Services

For continuous verification of the instrument performance test plates can be applied by the user and Performance Qualification services done by experienced Berthold Technologies personnel are available.
 

Cleanit Daily Injector Cleaning Solution

For proper maintenance of the injection system the Cleanit Daily solution is recommended for regular cleaning. Daily cleaning ensures that accuracy and precision as well as a long life time of the injectors will be maintained.

Applications

Calcium monitoring

Calcium monitoring in Arabidopsis using aequorin: Mark Kwaaitaal et al. (2011). Ionotropic glutamate receptor (iGluR)-like channels mediate MAMP-induced calcium influx in Arabidopsis thaliana. Biochemical Journal 440, 355–365 Read more

Circadian Rhythm

Luciferase Assay to monitor circadian clock genes in C. elegans: Olmedo et al. (2015). A High-Throughput Method for the Analysis of Larval Developmental Phenotypes in Caenorhabditis elegans. Genetics, doi: 10.1534/genetics.115.179242 Read more

Reporter Gene Assays

Luciferase Assay to monitor E2-ERα Signaling in cancer cells: Xiao et al. (2014). Induction of the CLOCK Gene by E2-ERα Signaling Promotes the Proliferation of Breast Cancer Cells. PlosONE, DOI: 10.1371/journal.pone.0095878 Read more

Luciferase Assays in HeLa cells: Jani et al. (2012). In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection. JoVE 61 Link to the video

Luciferase Reporter Assays in Ostreococcus tauri: Djouani-Tahri et al. (2011). An Eukaryotic LOV-histidine Kinase With Circadian Clock Function in the Picoalga Ostreococcus. The Plant Journal 65, 578-588 Read more

Dual-Luciferase assay in Arabidopsis: Liu et al. (2010). bZIP28 and NF-Y Transcription Factors are Activated by ER Stress and Assemble into a Transcriptional Complex to Regulate Stress Response Genes in Arabidopsis. The Plant Cell 22, 782-796 Read more

Dual luciferase reporter gene assay (DLR®): Tan et al. (2012). Integration of Regulatory Networks by NKX3-1 Promotes Androgen-Dependent Prostate Cancer Survival. Molecular and Cellular Biology 32, 399-414 Read more

Immunoassay

LIPS (luciferase immunoprecipitation systems) Assay: Burbelo et al. (2010). Rapid, Simple, Quantitative, and Highly Sensitive Antibody Detection for Lyme Disease. Clinical and Vaccine Immunology 17, 904-909 Read more

ATP determination

ADP and ATP assays: Belleannée et al.(2010). Role of purinergic signaling pathways in V-ATPase recruitment to apical membrane of acidifying epididymal clear cells. AJP Cellular Physiology 298, 817-830 Read more

Enzyme kinetics

Neuramidase Assay: Smee et al. (2010). Effects of the Combination of Favipiravir (T-705) and Oseltamivir on Influenza A Virus Infections in Mice. Antimicrobial Agents and Chemotherapy 54 (1), 126–133 Read more

CALUX® Assays

CALUX® Assays: Zhang et al. (2009). Bioanalytical characterization of dioxin-like activity in sewage sludge from Beijing, China. Chemosphere 75, 649-653 Read more

Cellular Chemiluminescence / Oxidative Burst

ROS assay: Cao et al. (2013). Mutations in FLS2 Ser-938 Dissect Signaling Activation in FLS2-Mediated Arabidopsis Immunity. PLoS Pathog 9(4), April 2013  Read More

ROS assay: Smith et al.(2009). The antiproliferative cytostatic effects of a self-activating viridin prodrug. Molecular Cancer Therapy 8, 1666 Read more

Cytotoxicity

CytoTox-Glo assay: Haid et al.(2009). Low pH-dependent Hepatitis C Virus Membrane Fusion Depends on E2 Integrity, Target Lipid Composition, and Density of Virus Particles. The Journal of Biological Chemistry, 284, 17657-17667 Read more


Further applications

Caspase assays - Cell proliferation - DNA probe assays - GPCRs / 7TM receptors - Kinase assays - Proteases

For detailed application information and selection of the best suited instrument please have a look to the Centro Selection Guide.

Download
Brochure
Format Size
Bioanalytic Instruments - Product Portfolio Brief overview scope of supply Bioanalytic (June 2014) de, en PDF 693.31 KB
Centro / Centro XS3 LB 960 Brochure 2012 de, en PDF 1.43 MB
Order Information Consumables & QC Test Devices Version Jan. 2017 de, en PDF 153.63 KB
Press & Media
Format Size
2012-04 (April 2012) Centro microplate luminometer NL Centro isuue Berthold Technologies Newsletter April 2012 de, en PDF 169.99 KB
2013-03 (March 2013) Sensitivity & Centro XS³ NL The Truth about Sensitivity & Centro XS³ Issue - Berthold Technologies Newsletter March 2013 de, en PDF 86.86 KB
Invitation 8th BioDetectors Conference Torino, Italy, 25th/26th September 2014 en PDF 298.47 KB

News about this product

This educational JoVE video explains in vitro transcription and capping of mRNA and subsequent transfection into HeLa cells and final measurement of luciferase activity in a Centro microplate luminometer

Read more
10.02.2015

PPIs represent a major target in drug discovery. Estimations reach up to half a Million of interactions in humans.

Read more
18.11.2013

The bioanalytic DR CALUX method is recommended by EU and is being routinely applied in many European countries to determine Dioxin contents in samples. The cell-based approach is significantly easier to perform and a lot more cost efficient than chemical methods.

Read more
14.11.2013