Mithras² LB 943 Monochromator Multimode Reader

Enhanced Versatility and Well-known Reliability

Mithras² LB 943 is a high-end microplate multimode reader based on monochromator technology with excellent performance. Renowned for its sensitivity and robustness especially in luminescence and BRET measurements the reader supports all important reading technologies including

  • Luminescence
  • BRET and BRET²
  • Fluorescence
  • FRET
  • Fluorescence Polarisation (FP)
  • UV/VIS Absorbance
  • AlphaScreen® and AlphaLISA®
  • Time-Resolved Fluorescence

In contrast to the usual kind of multi-technology instruments the Mithras multimode microplate reader has been designed with a proprietary optical system consisting of separate paths for different reading technologies (mDOPS), thus offering performances known from dedicated instruments. As each technology has its own demands on the optical system only separated light paths ensure that the needs for high sensitivity and a wide dynamic range are met with each technology.



Double Monochromators

Mithras² LB 943 employs two double monochromators for excitation and emission respectively. The monochromators are predominately meant to provide entire wavelength flexibility in absorbance and fluorescence measurents, yet they can be used for Time-Resolved Fluorescence and Luminescence measurements as well.

While a low f number and a high blocking ensure exceptional performance variable bandwidths and increments of 1 nm illustrate the versatility of the monochromators.

RFID-coded Filters

For applications requiring the high light transmission of filters, e.g. BRET and Fluorescence Polarization, the instrument can be equipped with filters. RFID-coding of the filters enable and secure their error-free usage.

Built-in Reagent Injectors

Up to 4 reagent injectors can be set up to dispense multiple activators or detection reagents at any time and with user-adjustable volumes during the measurement.

Berthold Technologies´ injectors are based on the proprietary JET Injection technology. Superior accuracy and precision is combined with excellent mixing performance. Variable injections speeds and "friendly" material enable even injection of live cells (e.g. in Aequorin based GPCR screening).

The injector tips can be located right in reading position enabling the measurement of very fast labels and reactions, e.g. Acridinium esters and Calcium flux, and to inject activators into a running kinetic.

Waste Pump

Excess fluids from priming and washing procedures are safely discarded via an automatic waste pump.

Temperature Control

A temperature controlled incubation area ensures stable conditions for the microplate whenever temperature sensitive enzymes or cells are in use. Condensation of lidded microplates is reduced.

Automatic Plate Height Adjustment (Z Optimization)

The plate height adjustment module detects the height of the plate used and automatically adjusts the measurement optics for it. There is no need any more for users to perform time-consuming Z-scans. Besides optimising the focus of the excitation beam it also ensures there will be no crosstalk from adjacent wells.





Certain configurations of this product are not available for sale in the U.S.A.

Detection Devices

2 low-noise photomultiplier tube operated in single photon counting mode, spectral range up to 850 nm
ultra-low-noise photomultiplier tube operated in single photon counting mode, spectral range up to 650 nm (HiSens position)
photo diode, spectral range 200 – 1000 nm

Excitation Sourceshalogen lamp
Xenon flash lamp
laser, 680 nm (Alpha Technology option)
Monochromatorf number: 2.7 (high light transmission)
variable bandwidth: 6 - 22 nm
increment: 1 nm
blocking: 10-6
FiltersRFID coded
Sample/Plate Formats6 – 1536 well plates
Terasaki plates
Petri dishes
filter membranes
Temperature Controlup to 45 °C
plate incubation area
Shaking3 modes, 3 velocities
Scanningup to 10,000 points per well
Injectionup to 4 JET injectors
variable volume 10 – 100 µL
variable speed
tips can be chosen to be located in 8 positions
Kinetics Operationminimum resolution 0.05 sec
maximum duration up to 7 days
Automationrobot integration
Luminescence<2 amol ATP per well
<2 zmol Firefly luciferase per well (equals less than 800 molecules)
Crosstalk5 x 10-6

<80 amol FITC per well (filter)
<700 amol FITC per well (monochromator)

Absorbanceaccuracy <2 % deviation (@ 2 OD, 405 nm)
precision <0.6 % CV (@ 2 OD, 405 nm)
Time-Resolved Fluor.<2 amol Europium per well
HTRF®dF high >1100 %
Fluorescence Polar.<4 mP SD (1 nM Fluorescein)
AlphaScreen®<1 ng Omni beads
Dynamic range>6 decades (PMT)
4 decades (photo diode)
Injectorsaccuracy better 98 % over entire volume range
precision better 98 % over entire volume range
Dimensions480 x 500 x 550 mm (w x h x d)
incl. injectors
Weight48 kg
OSWin XP, Win Vista, Win 7
RegulationsCE, UL, CSA

Specifications are subject without prior notice




Certain configurations of this product are not available for sale in the U.S.A.


The Mithras² LB 943 has been developped as consistent successor of the Mithras LB 940, thus all applications conducted with the latter can be performed with the Mithras² as well. Improvements in optics and the availability of monochromators even increase its usefulness for said applications.


Follow this link to the Mithras microplate reader applications

(simply go to the Applications tab once on the Mithras LB 940 main page)


Selected applications featuring the Mithras2 LB 943:


Bioluminescence Resonance Energy Transfer (BRET):

BRET to clarify Hormone Receptor Signaling Pathways: Abdulrahman, A. O., Ismael, M. A., Al-Hosaini, K., Rame, C., Al-Senaidy, A. M., Dupont, J., & Ayoub, M. A. (2016). Differential Effects of Camel Milk on Insulin Receptor Signaling – Toward Understanding the Insulin-Like Properties of Camel Milk. Frontiers in Endocrinology, 7, 4. Read More


Laser-induced BRET-Assay: Yimei Huang et al. (2015). Measuring the dynamics of cyclic adenosine monophosphate level in living cells induced by low-level laser irradiation using bioluminescence resonance energy transferJ. Biomed. Opt. 20(5), 051029 Read More

Immune Regulation: Il-Young Hwang, Chung Park, Kathleen Harrison, Cedric Boularan, Céline Galés, John H. Kehrl (2015) An Essential Role for RGS Protein/Gαi2 Interactions in B Lymphocyte–Directed Cell Migration and Trafficking. The Journal of Immunology 194 (5) 2128-2139 Read More


Energy Transfer Assays to access hormone receptor functions: Ayoub, M. A., Landomiel, F., Gallay, N., Jégot, G., Poupon, A., Crépieux, P., & Reiter, E. (2015). Assessing Gonadotropin Receptor Function by Resonance Energy Transfer-Based Assays. Frontiers in Endocrinology, 6, 130. Read More

Application Report
Format Size
Assessing Gonadotropin Receptor Function by Resonance Energy Transfer based Assays The Mithras2 was used to clarify the Gonadotropin receptor function using its versatile energy transfer measurement options. en PDF 1.7 MB
Differential Effects of Camel Milk on Insulin receptor Signaling The Mithras2 has been used to clarify the influence of camel milk on insulin activity as an important step to next generation diabetis therapies. en PDF 1.04 MB
Application Note
Format Size
Validation of the Mithras² LB 943 Monochromator Multimode Reader with the Transcreener® Fluorescence Intensity Assay Validation report, confirming the suitability of the Berthold Technologies Mithras² LB 943 Monochromator Multimode Reader for use with the Transcreener ® Fluorescence Intensity Assay. en PDF 1.01 MB
Validation of the Mithras² LB 943 Monochromator Multimode Reader with the Transcreener® GDP TR-FRET Red Assay Validation report, confirming the suitability of the Berthold Technologies Mithras² LB 943 Monochromator Multimode Reader for use with the Transcreener ® GDP TR-FRET Red Assay. en PDF 990.01 KB
Format Size
Bioanalytic Instruments - Product Portfolio Brief overview scope of supply Bioanalytic (June 2014) de, en PDF 693.31 KB
Filter RFID Data Sheet Dec2013 de, en PDF 207.25 KB
Flyer Mithras2 LB 943 de, en PDF 140.93 KB
Mithras2 LB 943 Brochure 2013 de, en PDF 2.17 MB
Order Information Consumables & QC Test Devices Version Jan. 2017 de, en PDF 153.63 KB
Press & Media
Format Size
2013-04 (April 2013) Injectors & Mithras² NL The Truth about Injectors & Mithras² Issue - Berthold Technologies Newsletter April 2013 de, en PDF 96.62 KB
Mithras2_graphics_fullpage_ad graphics for full page print advertisement (A4, letter and midadlantic); March 2013 en ZIP 15.1 MB
Mithras² multimode reader hi res image plate out en ZIP 28.81 MB
Press Release Mithras² Monochromator Microplate Reader April 2014 en DOCX 302.79 KB
Product Information
Format Size
models Mithras² available models Mithras2 LB 943 and their configurations (Nov 2013) de, en PDF 136.3 KB

News about this product

The modular multimode microplate TriStar² S LB 942 has successfully been certified for HTRF® by Cisbio. It is now the 3rd of Berthold´s portfolio of HTRF compatible microplate readers besides Mithras LB 940 and Mithras² LB 943. Read more
Watch the Select Science video coverage of the product presentations at Analytica 2014 in Munich about the Mithras² monochromator multimode microplate reader, the Read more

PPIs represent a major target in drug discovery. Estimations reach up to half a Million of interactions in humans.

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Have a look at a new publication describing the use of the ... Read more