TriStar² S LB 942 Modular Monochromator Multimode Reader

TriStar² Modular Multimode Reader with Monochromator

With TriStar² S the multi-technology microplate reader platform has been extended in its modularity, performance and user-friendliness by adding a double monochromator for wavelength selection and TRF reading technology.

  • Luminescence
  • BRET and BRET²
  • Fluorescence
  • FRET
  • UV/Vis Absorbance
  • Time-resolved fluorescence
  • Fluorescence Polarization (FP)
  • Top and Bottom Reading

The TriStar² S has been developed to support a fully modular approach. Any combination of reading technologies and options can be composed maintaining all possibilities of later upgrades.

The optical system ONE-4-ALL with a new revolutionary design is the first time a single optical system has been employed in a multi-technology reader that matches the performance of individual dedicated optical devices. The revolutionary 3D double monochromator offers full flexibility in wavelength selection.



Double Monochromator

TriStar² S  LB 942 employs a double monochromator for absorbance and fluorescecence excitation. The monochromator is predominately meant to provide entire wavelength flexibility and spectral scanning posibilities in absorbance and fluorescence measurements, yet they can be used for Time-Resolved Fluorescence measurements as well.

While a low f number and a high blocking ensure exceptional performance variable bandwidths and increments of 1 nm illustrate the versatility of the monochromators.

Top and Bottom Reading

TriStar2S  LB 942 can read microplates from top and from bottom. The latter is especially appropriate for the detection of adherent cells. Bottom reading is available for luminescence and fluorescence readout. Click here to learn more about the advantages of bottom reading.

Molecular Interaction assays

TriStar2S  LB 942 can handle multiple molecular interaction assays. Besides BRET and FRET assays, the instrument is certified for HTRF® TR-FRET interaction studies. For convenient single-label interaction studies, TriStar2S  LB 942 can be ordered with a fluorescence polarisation (FP) option.

Built-in Reagent Injectors

Up to 3 reagent injectors can be set up to dispense multiple activators or detection reagents at any time and with user-adjustable volumes during the measurement.JET injector

Berthold Technologies´ injectors are based on the proprietary JET Injection technology. Superior accuracy and precision is combined with excellent mixing performance. Variable injections speeds and "friendly" material enable even injection of live cells (e.g. in Aequorin based GPCR screening).
One of the injector tips is located in reading position enabling the measurement of very fast labels, e.g. Acridinium esters, and to inject activators into a running kinetic.

The ergonomic design of the housing provides front access of the injectors keeping the reagent reservoirs within reach and within eyesight. A through allows keeping the reagent bottles on ice or elevated temperatures – just as required.

Wash Plate

Excess fluids from priming and washing procedures are safely discarded via a specially designed wash plate.

Temperature Control

A temperature controlled microplate compartment ensures stable conditions whenever temperature sensitive enzymes or cells are in use. Condensation of lidded microplates is reduced.

Front Access of Reagents

The reagent reservoirs can be placed on the front for easy access and monitoring. A smoked see through lid protects the reagents from light. A base trough allows for the use of ice to keep the reagents cool.


In the TriStar² S any of reading technologies can be combined with each other and any of the options added.





Certain configurations of this product are not available for sale in the U.S.A.

Detection Deviceslow-noise photomultiplier tube operated in dual mode, spectral range 280 to 650 nm
photo diode, spectral range 200 – 1000 nm
Excitation SourcesXenon flash lamp

f number: 2.7 (high light transmission)
variable bandwidth: 4 - 22 nm
increment: 1 nm
blocking: 10-6

Sample/Plate Formats6 – 384 well plates
Terasaki plates
Petri dishes
filter membranes
Temperature Controlup to 45 °C
including Peltier cooling of photomultiplier
Shaking3 modes, 3 velocities
Scanningup to 10,000 points per well
Injectionup to 3 JET injectors
variable volume 10 – 100 µL
variable speed
Kinetics Operationminimum resolution 0.05 sec
maximum duration up to 7 days
Luminescence   <6 amol ATP per well
<3 zmol Firefly luciferase per well (equals less than 800 molecules)
Crosstalk   5 x 10-6

<65 amol FITC per well (96 well plate, filter)

<230 amol FITC per well (96 well plate, monochromator)

Fluorescence Polarization<5 mP SD (1nM Fluorescein)
TRF<5 amol Europium per well

dF high > 835 % (Filter)

dF high > 634% (Monochromator)

Absorbanceaccuracy <1 % deviation (@ 2.5 OD)
precision <0.3 % CV (@ 2.5 OD)
Dynamic range>6 decades (PMT)
4 decades (photo diode)
Injectorsaccuracy better 98 % over entire volume range
precision better 98 % over entire volume range
Dimensions   400 x 470 x 345 mm (w x d x h)
incl. injectors
OSWin XP, Win Vista, Win 7
Regulations   CE



ICE software

The TriStar² comes standard with the Research version of the ICE (Instrument Control and Evaluation) software. The ICE Research version supports ratio calculation for reporter gene applications, ADP and ATP determinations and kinetics functionality enzyme activities. Point-to-point curve fitting enables simple work with standard curves.
The ease of use during protocol creation, measurement and data export has been achieved with the wizard-driven and clearly structured software.
Advanced functionality can be added to the ICE software by upgrading to the Advanced version, e.g.

  •      extended reports for standard curve assays
  •      additional curve fit algorithms: linear regression, spline
  •      use of master and reference curves
  •      cut-off assay data reduction

Mikrowin software

This optional software combines operation and definition of instrument settings as well as data reduction and evaluation. The wells for measurement and injection can be selected independently. Various operations can be picked and placed in any order to accommodate for all different requirements of the assays to be performed.
Kinetic data reduction and graphical display of the respective curves help the user to judge the results.
Any type of ratio calculation, e.g. DLR®, or other mathematical formulas can be linked to every well individually.
Standard curve fitting and calculation of unknown samples is available for those users looking for quantification of their results (Advanced I and II).
All data can be exported in EXCEL® or ASCII formats. Instrument settings, user
signatures and file information are stored with the results.
The password system and the audit trail function of the Advanced I and II versions are important tools for data security.


For non-microplate shaped sample containers, e.g. Petri dishes, Terasaki plates and even filter membranes, special adapters are available to enable reading of this kind of lab ware in the Mithras.

Selected filter sets

For specific applications such as BRET, BRET² and ChromaGlo Berthold Technologies´ application specialists together with scientific users and reagent manufacturer´s scientists have put together optimised filters matching the reagents requirements and ensuring best performance.

BRET package4 filters for BRET and BRET² with extended transmission39350
ChromaGlo™2 filters specific for green and red emitting luciferases43544

Excitation and Emission Filters

More than 100 high quality filters for absorbance, fluorescence excitation and emission as well as luminescence are available. For details see optical filters.

Test Plates & QC Services

For continuous verification of the instrument performance test plates or test kits can be applied by the user and Performance Qualification services done by experienced Berthold Technologies personnel are available.

Cleanit Daily Injector Cleaning Solution

For proper maintenance of the injection system the Cleanit Daily solution is recommended for regular cleaning. Daily cleaning ensures that accuracy and precision as well as a long life time of the injectors will be maintained.


Reporter gene assays

Bacterial Luciferase assay in Ph. phosphoreum: Kamnev et al.(2013). Effects of americium-241 and humic substances on Photobacterium phosphoreum:
Bioluminescence and diffuse reflectance FTIR spectroscopic studies. Spectrochimica Acta Part A 100 (2013), 171–175 Read More

eGFP/dsRed Reporter assay in N. benthamiana leaves: Stauffer et al. (2010). Polypyrimidine tract-binding protein homologues from Arabidopsis underlie regulatory circuits based on alternative splicing and downstream control. The Plant Journal 64, 243–255 Read more

Dual Luciferase Reporter Assay: Juraver-Geslin et al. (2011). Barhl2 limits growth of the diencephalic primordium through Caspase3 inhibition of β-catenin activation. PNAS 108 (6), 2288-2293 Read more

Protease assays

Protease assay with fluorogenic peptide substrate: Jo et al. (2012). Development of α-Helical Calpain Probes by Mimicking a Natural Protein-Protein Interaction. J. Am. Chem. Soc, 2012 Oct 24;134(42) Read More


ELISA: Vogt et al. (2011). Poorly Neutralizing Cross-Reactive Antibodies against the Fusion Loop of West Nile Virus Envelope Protein Protect In Vivo via Fcγ Receptor and Complement-Dependent Effector Mechanisms. JOURNAL OF VIROLOGY, 11567–11580 Read more

Caspase assays

Caspase assay in clams: Strahl et al. (2010). Cell turnover in tissues of the long-lived ocean quahog Arctica islandica and the short-lived scallop Aequipecten opercularis. Marine Biology 157 (6), 1283-1292 Read more


LDH release (Cytotox Assay) Hamon et al. (2011). K+ Efflux Is Required for Histone H3 Dephosphorylation by Listeria monocytogenes Listeriolysin O and Other Pore-Forming Toxins. Infection and Immunity 79 (7), 2839-2846 Read more


GPCR oligomerization: Wade et al. (2011). Relationship between Homo-oligomerization of a Mammalian Olfactory Receptor and Its Activation State Demonstrated by Bioluminescence Resonance Energy Transfer. The Journal of Biological Chemistry 286, 15252-15259 Read more

Cell proliferation

ATPLite assay: Stavru et al. (2011). Cell Listeria monocytogenes transiently alters mitochondrial dynamics during infection. PNAS 108 (9), 3612-3617 Read more

Cell migration

Transwell permeability assay: Sume et al. (2010). Epithelial to Mesenchymal Transition in Gingival Overgrowth. The American Journal of Pathology 177 (1), 208–218 Read more

Cellular luminescence / Phagocytosis

Cellular chemiluminescence: Wan et al. (2010). A high throughput chemiluminescence method based on molecularly imprinted sol-gel films for determination of sibutramine. Advanced Material Letters 1 (2), 164-169 Read more

Enzyme kinetics

Proteasome assay: Jo et al. (2010). Natural Product Celastrol Destabilizes Tubulin Heterodimer and Facilitates Mitotic Cell Death Triggered by Microtubule-Targeting Anti-Cancer Drugs. PLoS ONE 5 (4), e10318 Read more

Reactive Oxygen Species (ROS)

ROS production in A549 cells: Zhang et al. (2008). Interactions between PBEF and oxidative stress proteins-a potential new mechanism underlying PBEF in the pathogenesis of acute lung injury. FEBS Letters 582 (13), 1802–1808 Read more


Transwell assay of fluorescent microsphere: Gali et al. (2010). In Vitro Evaluation of Viability, Integrity, and Inflammation in Genital Epithelia upon Exposure to Pharmaceutical Excipients and Candidate Microbicides. Antimicrobial Agents and Chemotherapy 54(12), 5105–5114 Read more

Further Applications

ATP determination  -  Apoptosis  -  Calcium monitoring  -  Chemotaxis  -  Cytokine analysis  -  ChromaGlo™ assays  -  DNA quantification  -  FRET  -  GPCRs / 7TM receptors  -  Ion channel studies  -  Kinase assays  -  Membrane potential  -  Nucleic acid quantification  -  Protein concentration  -  Protein-protein interactions  -  Receptor binding studies  -  SNP determination

Application Note
Format Size
Screening of GPCR-anatgonists using the Ca Mobilisation Assay Application Note describing usage of the Calcium Mobilisation Assay for GPCR-antagonist screening. de, en PDF 1009.46 KB
Validation of the TriStar² S LB 942 Monochromator Multimode Reader with the Transcreener® Fluorescence Intensity Assay Validation report, confirming the suitability of the Berthold Technologies TriStar² S LB 942 Monochromator Multimode Reader for use with the Transcreener ® Fluorescence Intensity Assay. en PDF 1.02 MB
Validation of the TriStar² S LB 942 Monochromator Multimode Reader with the Transcreener® GDP TR-FRET Red Assay Validation report, confirming the suitability of the Berthold Technologies TriStar² S LB 942 Monochromator Multimode Reader for use with the Transcreener ® GDP TR-FRET Red Assay. en PDF 934.01 KB
Format Size
LB 942 TriStar² & TriStar²S Broschure en PDF 4.58 MB
Order Information Consumables & QC Test Devices Version Jan. 2017 de, en PDF 153.63 KB
Press & Media
Format Size
Pictures of TriStar² S High Resolution Pictures en ZIP 6.99 MB
Press Release TriStar² LB 942 HTRF April 2015 en DOCX 126.13 KB

News about this product

The modular multimode microplate TriStar² S LB 942 has successfully been certified for HTRF® by Cisbio. It is now the 3rd of Berthold´s portfolio of HTRF compatible microplate readers besides Mithras LB 940 and Mithras² LB 943. Read more

TriStar² S multimode microplate reader has been enhanced in its versatility by adding TR-FRET functionality enabling the measurement of homogeneous time-resolved fluorescence assays.

Read more