Mithras LB 940 Multimode Microplate Reader

High Performance and Versatile Multilabel Reader

Mithras LB 940 is a modular and reliable microplate multimode reader with outstanding performance. Renowned for its sensitivity and robustness especially in luminescence and BRET measurements the reader supports all important reading technologies including

  • Luminescence
  • BRET and BRET²
  • Fluorescence (top and bottom)
  • FRET
  • Fluorescence Polarisation (FP)
  • UV/VIS Absorbance
  • AlphaScreen® and AlphaLISA®
  • Time-Resolved Fluorescence
  • HTRF®


“Based on my experience with the Mithras LB 940, I feel that this equipment offers advantages over those of its competitors that would be worth to directly communicate to researchers (in particular its incredible robustness).”

Nikolaus Heveker, Hôpital Sainte-Justine, Montréal

See what other users are saying about this product

In contrast to the usual kind of multi-technology instruments the Mithras multimode microplate reader has been designed with a proprietary optical system consisting of optimised paths for each reading technology (DOPS). So, Mithras offers performances known from dedicated instruments. As each technology has its own demands on the optical system only separated light paths ensure that the needs for high sensitivity and a wide dynamic range are met with each technology.

Tabs

Features

Built-in Reagent Injectors

Up to 4 reagent injectors can be set up to dispense multiple activators or detection reagents at any time and with user-adjustable volumes during the measurement.
Berthold Technologies´ injectors are based on the proprietary JET Injection technology. Superior accuracy and precision is combined with excellent mixing performance. Variable injections speeds and "friendly" material enable even injection of live cells (e.g. in Aequorin based GPCR screening).

Three of the injector tips are located in reading position enabling the measurement of very fast labels, e.g. Acridinium esters, and to inject activators into a running kinetic.

Waste Pump

Excess fluids from priming and washing procedures are safely discarded via an automatic waste pump.

Temperature Control

A temperature controlled microplate compartment  ensures stable conditions whenever temperature sensitive enzymes or cells are in use.

Automatic Plate Height Adjustment (Z Optimization)

The plate height adjustment module detects the height of the plate used and automatically adjusts the measurement optics for it. There is no need any more for users to perform time-consuming Z-scans. Besides optimising the focus of the excitation beam it also ensures there will be no crosstalk from adjacent wells.

Specifications
Detection Devices

low-noise photomultiplier tube operated in single photon counting mode, spectral range up to 850 nm (dependent on model)
photo diode, spectral range 200 – 1000 nm

Excitation Sourceshalogen lamp
Xenon flash lamp (TRF, HTRF®, UV absorbance)
laser, 680 nm (AlphaScreen® option)
Sample/Plate Formats6 – 1536 well plates
Terasaki plates
Petri dishes
filter membranes
Temperature Controlup to 45 °C
including Peltier cooling of photomultiplier
Shaking3 modes, 3 velocities
Scanningup to 10,000 points per well
Injectionup to 4 JET injectors
variable volume 10 – 100 µL
variable speed
Kinetics Operationminimum resolution 0.05 sec
maximum duration up to 7 days
Automationrobot integration
stacker unit
Performance 
Luminescence<6 amol ATP per well
<3 zmol Firefly luciferase per well (equals less than 800 molecules)
Crosstalk5 x 10-6
Fluorescence<0.3 fmol FITC per well
Absorbanceaccuracy <2 % deviation (@ 2 OD, 405 nm)
precision <0.6 % CV (@ 2 OD, 405 nm)
Time-Resolved Fluor.<2 amol Europium per well
HTRF®dF high >900 %
Fluorescence Polar.4 mP SD (1 nM Fluorescein)
AlphaScreen®<12.5 ng standard beads
Dynamic range>6 decades (PMT)
4 decades (photo diode)
Injectorsaccuracy better 98 % over entire volume range
precision better 98 % over entire volume range
Miscellaneous 
Dimensions472 x 494 x 374 mm
incl. injectors
OSWin XP, Win Vista, Win 7
RegulationsCE, UL, CSA

 

Accessories

Mikrowin software

The Windows® based software combines operation and definition of instrument settings as well as data reduction and evaluation.
The wells for measurement and injection can be selected independently. Various operations can be picked and placed in any order to accommodate for all different requirements of the assays to be performed.
Kinetic data reduction and graphical display of the respective curves help the user to judge the results.
Any type of ratio calculation, e.g. DLR®, or other mathematical formulas can be linked to every well individually.
Standard curve fitting and calculation of unknown samples is available for those users looking for quantification of their results (Advanced I and II).
All data can be exported in EXCEL® or ASCII formats. Instrument settings, user
signatures and file information are stored with the results.
The password system and the audit trail function of the Advanced I and II versions are important tools for data security.

Stacker

An optional microplate stacker is available when throughput and walk away automation are of interest. The Stacker unit is available with 25 or 50 plate stacks. The unique Re-Stack functionality of the stacker enables monitoring of e.g. gene expression over extended periods of time with multiple plates.

Robot Integration

Specially designed for but not restricted to the use in HTS departments of drug discovery companies the robot access module allows for uncomplicated integration of the Mithras into any type of lab automation system. Software and hardware easily integrate into existing robotic or liquid handling systems.

Adapters

For non-microplate shaped sample containers, e.g. Petri dishes, Terasaki plates and even filter membranes, special adapters are available to enable reading of this kind of lab ware in the Mithras.

Selected filter sets

For specific applications such as BRET, BRET² and ChromaGlo Berthold Technologies´ application specialists together with scientific users and reagent manufacturer´s scientists have put together optimised filters matching the reagents requirements and ensuring best performance.

 

BRET package4 filters for BRET and BRET²39350
BRET high efficiency2 filters for BRET with improved transmission53431
BRET² high efficiency2 filters for BRET² with improved transmission53432
ChromaGlo™2 filters specific for green and red emitting luciferases43544
ChromaGlo™ (red enh.PMT)2 filters specific for green and red emitting luciferases

43541 and 55133

 

Excitation and Emission Filters

More than 100 high quality filters for absorbance, fluorescence excitation and emission as well as luminescence are available.
For details see the Optical Filters

Test Plates & QC Services

For continuous verification of the instrument performance test plates or test kits can be applied by the user and Performance Qualification services done by experienced Berthold Technologies personnel are available.

Cleanit Daily Injector Cleaning Solution

For proper maintenance of the injection system the Cleanit Daily solution is recommended for regular cleaning. Daily cleaning ensures that accuracy and precision as well as a long life time of the injectors will be maintained.

Applications

Reporter Gene

Dual-Colour-Luciferase Assay (Chroma-GloTM) in yeast: Shimada et al. (2012). Schizosaccharomyces pombe reporter strains for relative quantitative assessment of heterochromatin silencing. Yeast, 29: 335–341. doi: 10.1002/yea.2913 Read More

 

Dual-Luciferase Assay in Antirrhinum Petal Cells: Fujino et al. (2011). Temperature controls nuclear import of Tam3 transposase in Antirrhinum. Plant Journal 65, 146-155 Read more

 

Dual luciferase reporter assay (DLR®) in HEK cells: Fiesel et al. (2012). TDP-43 regulates global translational yield by splicing of exon junction complex component SKAR. Nucleic Acids Research 40 (6), 2668-2682 Read more

Second Messenger

HTRF® cAMP and IP1: Schmidt et al. (2011). Selective orthosteric free fatty acid receptor 2 (FFA2) agonists: identification of the structural and chemical requirements for selective activation of FFA2 versus FFA3. The Journal of Biological Chemistry 286, 10628-10640  Read more

 

cAMP with CFP/YFP FRET: Nikolaev et al. (2006). Molecular basis of partial agonism at the neurotransmitter alpha2A-adrenergic receptor and Gi-protein heterotrimer. The Journal of Biological Chemistry 281, 24506-24511 Read more

BRET and BRET²

BRET to clarify receptor oligomerization: TH Lan, Q Liu, C Li, G Wu, J Steyaert, NA Lambert (2015). BRET evidence that β2 adrenergic receptors do not oligomerize in cells. Nature Scientific Reports 5 Read more

Estrogen and GnRH signaling: Rebecca J. Chasona, Jung-Hoon Kangb, Sabrina A. Gerkowiczc, Maria L. Dufaub, Kevin J. Cattd, James H. Segarsa (2015). GnRH agonist reduces estrogen receptor dimerization in GT1-7 cells: Evidence for cross-talk between membrane-initiated estrogen and GnRH signaling. Molecular and Cellular Endocrinology 404 (15) 67–74 Read more

 

Dopamin receptor pathways: E. Martínez-Pinillaa, A.I. Rodríguez-Pérezb, G. Navarroc, D. Aguinagac, E. Morenoc, J.L. Lanciegoa, J.L. Labandeira-Garcíab, R. Francoc, d, 2 (2015) Dopamine D2 and angiotensin II type 1 receptors form functional heteromers in rat striatum. Biochemical Pharmacology 96 (15) 131–142 Read more

 

Functional Ligand Binding Assay : Journé et al. (2014). N1-linked melatonin dimers as bivalent ligands targeting dimeric melatonin receptors. Med. Chem. Commun., 2014,5, 792-796 Read More

 

Functional Ligand Binding Assay : Berchiche et al. (2011). Different Effects of the Different Natural CC Chemokine Receptor 2b Ligands on beta-Arrestin Recruitment, Gi Signaling, and Receptor Internalization. Molecular Pharmacology 79, 488–498 Read More

 

Functional Ligand Binding Assay to GPCRs: Kamal et al. (2009). Improved donor/acceptor BRET couples for monitoring β-arrestin recruitment to G protein-coupled receptors. Biotechnology Journal, Methods and Advances 4 (9), 1337–1344 Read More

Cytotoxicity

CellTiter-Glo, CellTiter-Blue, Cytotox-ONE and Apo-ONE: Gaunitz et al. (2003). HTS compatible assay for antioxidative agents using primary cultured hepatocytes. ASSAY and Drug Development Technologies 1 (3), 469-77 Read more

 

Superoxide assay with DHE: Patschan et al. (2008). Mapping mechanisms and charting the time course of premature cell senescence and apoptosis: lysosomal dysfunction and ganglioside accumulation in endothelial cells. AJP Renal Physiology 294 (1), 100-9 Read more

ATP determination

Luciferin-based ATP assay: Terhaz et al. (2010). Mislocalization of mitochondria and compromised renal function and oxidative stress resistance in Drosophila SesB mutants. Physiolical Genomics 41, 33-41 Read more

Calcium monitoring

Intracellular Calcium assay with Fura-2: Kawasaki et al. (2010). Protein Kinase C-induced Phosphorylation of Orai1 Regulates the Intracellular Ca2+ Level via the Store-operated Ca2+ Channel. The Journal of Biological Chemistry 285, 25720-25730 Read more

AlphaScreen®/ ADLOC

ADLOC Assay: Niebel et al. (2010). ADLOC: An Aptamer-Displacement Assay Based on Luminescent Oxygen Channeling. Chemistry 16 (36), 11100-11107 Read more

Caspase assay

Caspase assay with DEVD-amc: Eisele et al. (2011). APO010, a synthetic hexameric CD95 ligand, induces human glioma cell death in vitro and in vivo. Neuro-Oncology 13 (2), 155–164 Read more

Kinase assay

FRET protein kinase assay with EDANS/DABCYL: Gratz et al. (2010). A FRET-based microplate assay for human protein kinase CK2, a target in neoplastic disease. Journal of Enzyme Inhibition and Medicinal Chemistry 25 (2), 234–239 Read more

Protease assay

Protease assay with Bodipy FL casein substrate: Paul et al. (2013). Induction of Protease Release of the Resistant Diatom Chaetoceros didymus in Response to Lytic Enzymes from an Algicidal Bacterium. PLoS One. 2013; 8(3): e57577 Read More

Cellular luminescence / Phagocytosis

Lucigenin-enhanced chemiluminescence assay of neutrophils: Mauch et al. (2007). Chronic Granulomatous Disease (CGD) and Complete Myeloperoxidase Deficiency Both Yield Strongly Reduced Dihydrorhodamine 123 Test Signals but Can Be Easily Discerned in Routine Testing for CGD. Clinical Chemistry 53 (5), 890-896 Read more

Immunoassays

Chemiluminescence Immunoassay: Bogan et al. (2007). Constitutive Steroidogenesis in Ovine Large Luteal Cells May Be Mediated by Tonically Active Protein Kinase A1. Biology of Reproduction 77 (2), 209-216 Read more

 

Chemiluminescence Immunoassay with Acridinium Ester: Adamczyk et al.(2006). Choline Concentration in Normal Blood Donor and Cardiac Troponin–Positive Plasma Samples. Clinical Chemistry 52 (11), 2123-2124 Read more

SNP determination

SNP assay: Okamoto et al. (2005). Simple SNP typing assay using a base-discriminating fluorescent probe. Molecular BioSystems 2, 122–127 Read more

Apoptosis

Calpain assay with AFC: Lee et al. (2007). Cadmium-induced ceramide formation triggers calpain-dependent apoptosis in cultured kidney proximal tubule cells. AJP Cell Physiology 293, 839–847 Read More

Enzyme kinetics

Proteasome activity assay with MCA: Hubert-Buron et al. (2006). Glutamine Pretreatment Reduces IL-8 Production in Human Intestinal Epithelial Cells by Limiting IkBa Ubiquitination. Journal of Nutrition 136 (6), 1461-1465 Read More

 

Proteasome and Cathepsin B activity: Fujita et al. (2007). α-Synuclein Stimulates Differentiation of Osteosarcoma Cells: RELEVANCE TO DOWN-REGULATION OF PROTEASOME ACTIVITY. The Journal of Biological Chemistry, 282, 5736-5748 Read more

Cell density / Cell proliferation

Diatom growth based on Chlorophyll a fluorescence: Paul et al. (2013). Induction of Protease Release of the Resistant Diatom Chaetoceros didymus in Response to Lytic Enzymes from an Algicidal Bacterium. PLoS One. 2013; 8(3): e57577  Read More

 

Cell density assay with crystal violet: Nantia et al. (2012). Effects of the Methanol Extract of Basella alba L (Basellaceae) on Steroid Production in Leydig Cells. Andrologia 44 (1), 38–45 Read More

Nucleic acid quantification

DNA quantification: Little et al. (2004)
Poly- amino ester-containing microparticles enhance the activity of nonviral genetic vaccines. PNAS 101 (26), 9534–9539 Read More

Protein quantification

Protein quantification with Coomassie Brilliant Blue: Mesnagea et al. (2013)
Ethoxylated adjuvants of glyphosate-based herbicides are active principles of human cell toxicity. May 06 2013, only electronically available.  Read More

Binding Assay

Peptide binding with Fluorescence Polaristaion: Burgevin et al. (2008) A Detailed Analysis of the Murine TAP Transporter Substrate Specificity. PLoS ONE 3, 6 Read more

Further Applications

Chemotaxis - ChromaGlo™ assays - Cytokine analysis - DNA probe assays - Ion channel studies - GPCRs / 7TM receptors - Membrane potential - Proteases - Protein concentration - Protein-protein interactions

 

Download
Application Report
Format Size
AN LB940 001c BRET 7TM Receptor A Functional BRET Assay for 7TM Receptors Using the Mithras de, en PDF 312.01 KB
AN LB940 002a AlphaScreen cAMP Mithras LB 940 Multimode Plate Reader Used for AlphaScreen Signal Detection: Measurement of cAMP Production Induced by a G-protein Coupled Receptor de, en PDF 322.87 KB
AN LB940 003c FP Estrogen Recpetor Characterization of the interaction between recombinant estrogen receptor alpha and transcriptional coactivator SCR-1 NR-2 binding motif through fluorescence polarization study with Mithras LB 940 de, en PDF 312.59 KB
AN LB940 004b BRET Receptor Dynamics Bioluminescence Resonance Energy Transfer (BRET)-based studies of receptor dynamics in living cells with Berthold’s Mithras de, en PDF 334.85 KB
AN LB940 005c BRET in receptor protein interactions Bioluminescence Resonance Energy Transfer (BRET) as a means of monitoring dynamic receptor-protein interactions in living cells measured on LB 940 Mithras de, en PDF 403.46 KB
AN LB940 006a BRET Basics for GPCRs Basic Considerations for Bioluminescence Resonance Energy Transfer (BRET) Assays for G-protein coupled receptor protein interactions in living cells de, en PDF 321.96 KB
AN LB940 007c Calcium measurements with Fura Calcium monitoring with the Mithras LB 940 multimode plate reader de, en PDF 1.19 MB
AN LB940 008a ADP Quest HS kinetic assay ADP Quest™ HS kinetic assay with Mithras LB 940 de, en PDF 295.14 KB
AN LB940 009a PhopsphoQuest ELISA kinase assay PhosphoQuest™ cell based ELISA kinase assay with Mithras LB 940 de, en PDF 369.64 KB
AN LB940 010a HitHunter cAMP HS+ assay HitHunter™ cAMP HS+ assay with Mithras LB 940 de, en PDF 515.21 KB
AN LB940 011a PathHunterBetaArrestin PathHunter™ ß-arrestin assay with Mithras LB 940 de, en PDF 255.02 KB
AN LB940 012a PathHunterFlashBetaArrestin PathHunter™ ß-arrestin flash assay with Mithras LB 940 de, en PDF 346.16 KB
AN LB940 013a PathHuntersecretion PathHunter™ ß-arrestin assay with Mithras LB 940 de, en PDF 274.24 KB
AN LB940 014a Cell viability Cell viability assays with Mithras LB 940 de, en PDF 1.48 MB
AN LB940 015a HTplex cAMP IP1 cAMP/IP-One HTplex cell-based experiment performed on Mithras LB 940 using HTRF® technology de, en PDF 686.56 KB
AN LB940 016a High efficiency filters Comparison of filter sets for BRET1 assays: ß-arrestin2 (ßARR2) recruitment to the vasopressin V2 receptor de, en PDF 187.57 KB
AN LB940 017a Transil Assay TRANSIL® Brain Absorption Assay with Mithras LB 940 de, en PDF 251.3 KB
AN LB940 018a Hudson Cytochrome Assay Measuring Cytochrome P450-3A4 Inhibition Assay on Hudson Robotics’ VaryScreen ADME Equipped with Berthold Technologies’ Mithras de, en PDF 286.47 KB
AN LB940 019a Plant ROS Measurement of Reactive Oxygen Species (ROS) in Arabidopsis and N. benthamiana plants de, en PDF 333.04 KB
AN LB940 020b FRET FRET as a Tool to Study G-protein Coupled Receptor Oligomerization in HEK Cells de, en PDF 397.85 KB
AN940_021_Photosensitive_DNA Photochemical process for detection of nucleic acids in vitro de, en PDF 240.7 KB
Brochure
Format Size
Bioanalytic Instruments - Product Portfolio Brief overview scope of supply Bioanalytic (June 2014) de, en PDF 693.31 KB
Filter Data Sheet Dec 2013-V3 de, en PDF 604.67 KB
Mithras LB 940 Brochure 2010 de, en PDF 3.8 MB
Mithras LB 940 Overview Models & Options 2009 de, en PDF 132.22 KB
Order Information Consumables & QC Test Devices Version Jan. 2017 de, en PDF 153.63 KB
Press & Media
Format Size
2013-02 (February 2013) Optical Filters & Mithras NL The Truth about Optical Filters & Mithras Issue - Berthold Technologies Newsletter Feb. 2013 de, en PDF 98.05 KB
Invitation 8th BioDetectors Conference Torino, Italy, 25th/26th September 2014 en PDF 298.47 KB

News about this product

The modular multimode microplate TriStar² S LB 942 has successfully been certified for HTRF® by Cisbio. It is now the 3rd of Berthold´s portfolio of HTRF compatible microplate readers besides Mithras LB 940 and Mithras² LB 943. Read more
01.10.2015

Mithras LB 940 is a modular and reliable microplate multimode reader with outstanding performance. Scientists who use the instrument daily confirm this.

This is what has been said on SelectScience:

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10.06.2014

PPIs represent a major target in drug discovery. Estimations reach up to half a Million of interactions in humans.

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18.11.2013

Have a look at a new publication describing the use of the Mithras LB 940 multilabel microplate reader in a Chroma-GloTM reporter gene assay.

Read more
15.02.2013