Bioanalytic07.08.2013

NightOWL and TriStar being used in stem cell research

The anticancerous effect of iPS-cell–derived myeloid/macrophage line in vitro, in vivo and in situ is described by Koba et al. (2013) in their publication Therapeutic Effect of Human iPS-Cell–Derived Myeloid Cells Expressing IFN-β against Peritoneally Disseminated Cancer in Xenograft Models.

NUGC-4 cells (5×103 cells/well) expressing luciferase were cultured with or without iPS-ML (2.5×104 cells/well) in 96-well flat-bottomed culture plates. After a 3-day culture, 50 µL/well of luciferase substrate solution was added, and luminescence was measured on a TriStar microplate reader.

SCID mice were i.p. injected with the cancer cells (5×106 cells/mouse). Mice in the treatment group were injected with iPS-ML according to the indicated schedule, and cancer cell growth was monitored in mice by luminescence imaging analysis in the NightOWL.

Green fluorescence protein (GFP)-expressing NUGC-4 cells (5×106 cells/mouse) were injected into the peritoneal cavity of SCID mice. Mice were sacrificed the following day and subjected to fluorescence analysis to macroscopically detect the location of NUGC-4 tumors and iPS-ML on a NightOWL II imager. NUGC-4/GFP was detected with 475 nm excitation and 520 nm emission filters, and PKH26-labeled iPS-ML was detected with 550 nm excitation and 600 nm emission filters.

Here is the link to the article

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0067567