Bioanalytic15.12.2014

NightSHADE publications in Nature and Plant Cell

Photosynthetic entrainment of the Arabidopsis circadian clock is the title of the Nature publication by Michael Haydon et al. (Department of Plant Sciences, University of Cambridge) describing that the rhythmic endogenous sugar signals can entrain circadian rhythms in Arabidopsis by regulating circadian clock gene expression early in the photoperiod to define a ‘metabolic dawn’. By inhibiting photosynthesis it is demonstrated that endogenous oscillations of sugars provide metabolic feedback to the circadian oscillator through the morning-expressed PSEUDO RESPONSE REGULATOR 7 (PRR7) and identify that prr7 mutants are insensitive to the effects of sugar on circadian period. The NightSHADE is used for the circadian luciferase measurements and for Delayed Fluorescence measurements to monitor ROS abundance. Here is the link to the Nature article: http://www.nature.com/nature/journal/v502/n7473/full/nature12603.html

Zhang et al. (State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University) published Jasmonate-Activated MYC2 Represses ETHYLENE INSENSITIVE3 Activity to Antagonize Ethylene-Promoted Apical Hook Formation in Arabidopsis in Plant Cell. The authors show that MYC2 binds to the promoter of an F-box gene, EIN3 BINDING F-BOX PROTEIN1, to induce its expression and thus promote EIN3 degradation and that MYC2 physically interacts with EIN3 and inhibits its DNA binding activity. Follow this link to the Plant Cell article: http://www.ncbi.nlm.nih.gov/pubmed/24668749