Junior LB 9509 Portable Tube Luminometer

The Portable Luminometer

Junior is a small portable tube luminometer designed for all common applications using glow-type bioluminescent and chemiluminescent reagents. It can be used in a number of diverse fields of application including

  • biomedical research
  • clinical diagnostics
  • hygiene monitoring
  • process control in biotech
  • environmental monitoring of water quality

Excellent performance, its small size and low weight paired with battery operation make the Junior a highly versatile instrument which can be used in any location – the laboratory, on site or outdoors.

 

Tabs

Features

Sensitivity

The detection unit of the Junior consists of a state-of-the art ultra fast single photon counting photomultiplier with low noise. For demanding applications a “high sensitivity” model with a specifically selected ultra low noise detector is available driving the detection limit down to less than 50 amol ATP.

Dynamic Range

Berthold Technologies single photon counting electronics and selected photomultiplier tubes with low noise and high saturation level ensure a wide dynamic range. The user will benefit by being able to measure low signals as well as high ones without the need to change any settings.

Reliability

A stringent selection of detectors ensures a neglectable day-to-day variation of instruments, thus providing a high reliability of the measurements.

Versatility

The unique design of the measuring chamber allows the use of a variety of different sample tubes and sizes. Besides the traditional lumi vials, micro centrifuge tubes (Eppendorf cups) and capped 4 mL vials can be used.

Independency

The Junior can be operated independently from a mains supply for many hours as it is equipped with rechargeable batteries. In the lab the instrument can be connected to a mains supply thereby automatically recharging the batteries.

Portability

With a weight of only 2 kg and its small size the Junior can be carried  and taken to the sampling location for measurements on site. A metal transport case providing space for the Junior and accessories is an ideal solution for outdoor use.

Ease of Use

The lean and dedicated software is operated through a membrane keypad. The well arranged graphical display shows selected protocol, sample location and number and the RLU value of the measurement. In addition a set consisting of a red, a yellow and a green LED unmistakably indicates whether a signal and thus a sample is above an upper limit (red), below a lower limit (green) or in between (yellow).

Economical

The graphic display is illuminated only when the instruments is in use. As soon as the instrument is idle the illumination is turned off to save energy.

Smart Memory

Up to 2,000 measurement results will be stored in the instrument´s memory. Criteria like time of measurement and measurement location may be used to search for results in order to review them. The same criteria may be applied to select results for being downloaded to a computer

Specifications
Detection Devicesphotomultiplier tube operated in single photon counting mode, spectral range 380 – 630 nm
Sample Formatslumi vials
Eppendorf cups
4 mL vials
Performance 
Luminescence   <3 fmol ATP per vial (standard model)
<50 amol ATP per vial (high sensitivity model)
Miscellaneous 
Dimensions   150 x 280 x 170 mm (W x D x H)
Weight2 kg
Regulations   CE, UL
Operation Temperature15 – 35 °C

 

Accessories

WinTerm Software

The PC software arranges the data properly in an easy-to-comprehend layout and transfers the data to spreadsheet softwares like EXCEL.

Transport Case

For outdoor use the transport case is the ideal storage and transport solution. Foamed interior compartments provide spaces for the instrument and various accessories.

Lumi Vials

Polystyrene vials are available in various sizes. The 3 mL vials are gamma irradiated to provide sterile conditions for cell cultures or the use of primary cells.

  •      Lumi vials 5 mL, 12 x 75 mm
  •      Lumi vials 3.5 mL, 12 x 55 mm
  •      Lumi vials 3 mL, 12 x 47 mm, irradiated

Test Devices & QC Services

For continuous verification of the instrument performance test tube can be applied by the user and Performance Qualification services done by experienced Berthold Technologies personnel are available.

Luminescence Test Tube

PQ Services

Applications

Reporter gene assays

Luciferase assay in barley: Kumar et al. (2011). Cloning and characterization of barley caryopsis FCA. Physiologia Plantarum 143, 93–106 Read more

Luciferase assay in Jurkat T lymphocytes: Stevens et al. (2010). T-cell receptor early signalling complex activation in response to interferon-α receptor stimulation. Biochemical Journal 428, 429–437 Read More

Dual Luciferase Assay (DLR®): Torrano et al. (2011). ETV6-RUNX1 promotes survival of early B lineage progenitor cells via a dysregulated erythropoietin receptor. Blood 118 (18), 4910-4918 Read more

Chemiluminescence

Chemiluminescence: Sorouraddin et al. (2011). Direct chemiluminescence determination of penicillin G potassium and a chemometrical optimization approach. Luminescence 26, 622–628 Read more

Receptor Binding Studies

Thyrotropin receptor binding study using CRE luciferase: Lado-Abeal et al. (2011). A Family with Congenital Hypothyroidism Caused by a Combination of Loss-of-Function Mutations in the Thyrotropin Receptor and Adenylate Cyclase-Stimulating G Alpha-Protein Subunit Genes. Thyroid 21 (2), 103-109 Read more

Reactive Oxygen Species (ROS)

Measurement of free oxygen radicals in scar tissue:  Soybir et al. (2012). Delayed cutaneous wound healing in aged rats compared to younger ones. International Wound Journal in press Read more

ROS: Karadeniz Cerit et al. (2012). The antifibrotic drug halofuginone reduces ischemia/reperfusion-induced oxidative renal damage in rats. Journal of Pediatric Urology In press Read more

Water and Soil Toxicity

Luciferase biosensors for heavy metals: Amaro et al. (2011). Whole-cell biosensors for detection of heavy metal ions in environmen-tal samples based on metallothionein promoters from Tetrahymena thermophila. Microbial Biotechnology 4, 513–522 Read more

Field Testing of Groundwater: Siegfried et al. (2012)
Field Testing of Arsenic in Groundwater Samples of Bangladesh Using a Test Kit Based on Lyophilized Bioreporter Bacteria. Environmental Science & Technology 46 (6), 3281-3287 Read more

ATP Determination

ATP in mammalian cancer cells: Agbor et al. (2011). Small Ubiquitin-related Modifier (SUMO)-1 Promotes Glycolysis in Hypoxia. Journal of Biological Chemistry 286, 4718-4726 Read more

 

Further Applications

Monitoring of luminescent bacteria - Enzyme activity - LIA (luminescent immuno assay) - hygiene monitoring - Cell viability - DNA probe assay

Download
Brochure
Format Size
Bioanalytic Instruments - Product Portfolio Brief overview scope of supply Bioanalytic (June 2014) de, en PDF 693.31 KB
Junior LB 9509 Brochure 2009 de, en PDF 784.26 KB
Order Information Consumables & QC Test Devices Version Jan. 2017 de, en PDF 153.63 KB

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