Lumat³ LB 9508 Single Tube Luminometer

Setting Standards for Tube Luminometers

Already in its 3rd generation the Lumat³ offers the functionality, versatility and superior performance you can expect from an instrument developed by Berthold Technologies.

Maintaining the achievements of the prior generations like true single photon counting detector, frictionless JET injectors and the sample turret the Lumat³ sets a new standard with the ICE user software.

Wizard guided protocol definition, intuitive operation and especially today´s expectations for data transfer and storage are achievements every user will benefit from.

Tabs

Features

Sensitivity

True photon counting technology coupled with selected low noise photomultipliers and an optimised optical design is the only accepted way to achieve a low and stable background. This is a major parameter for high sensitivity in a luminescence measuring system enabling detection of extremely low amounts of analyte

  •      less than 1 amol ATP per vial
  •      less than 0.5 zmol Firefly luciferase per vial

ICE Software

The ICE software Research version supports ratio calculation for reporter gene applications, ADP and ATP determinations and kinetics functionality enzyme activities. Point-to-point curve fitting enables simple work with standard curves.
The ease of use during protocol creation, measurement and data export has been achieved with the wizard-driven and clearly structured software.

Unique Sample Loading – the Turret

The measurement chamber is designed to enable rotating sample transport. You can load a second sample while the first one is being measured, thus saving time which results in throughputs approaching those of an automatic instrument. The special design of measurement chamber provides superior light collection efficiency and contributes to the outstanding sensitivity.

Large Dynamic Range

The Lumat³ is able to count up to 25 Mio pulses per second equivalent to a dynamic range spanning 7 orders of magnitude. As no additional adjustments (such as gain and high voltage setting) are needed the Lumat³ offers convenience and security for every measurement over the entire life of the instrument.

Eppendorf Cups and Lumi Vials

In addition to all types of 12 mm Lumi vials the Lumat³ accepts Eppendorf tubes in 1 mL to 2 mL sizes.

Built-in Reagent Injectors

The instrument can be equipped according to needs with either no or 2 reagent injectors with variable volume in JET injection technology. You have the choice between a 100 µL and a 300 µL size injector. Superior accuracy and precision is combined with excellent mixing performance. Variable injections speeds and "friendly" material enable even injection of live cells (e.g. in Aequorin based GPCR screening).
The injector tips are located in reading position enabling the measurement of very fast labels, e.g. Acridinium esters, and to inject activators into a running kinetic.

Precision & Accuracy

Precise mechanics and the intrinsic stability of photon counting technology guarantee unrivalled repeatability of measurements. The instrument´s performance is stable over years. Time-consuming daily calibration is thus unnecessary and you can concentrate on other and more important duties.
Like all Berthold Technologies instruments the Lumat³ comes to your laboratory checked against a certified light source. With this reassurance you are able to work with accurate, traceable and comparable results.

Specifications
Detection Deviceslow-noise photomultiplier tube operated in single photon counting mode, spectral range 380 – 630 nm
Sample Formatslumi vials
Eppendorf cups
Sample Loadingrotor with measurement and load/unload position
Injectionup to 2 JET injectors
variable volume 10 – 100 µL or 25 – 300 µL
variable speed
Kinetics Operationminimum resolution 0.05 sec
maximum duration up to 7 days
Performance 
Luminescence<5 amol ATP per vial (standard models)
<0.5 amol ATP per vial (high sensitivity model)
<5 zmol Firefly luciferase per vial (standard model)
<1 zmol Firefly luciferase per vial (high sensitivity model)
Injectorsaccuracy better 98 % over entire volume range
precision better 98 % over entire volume range
Miscellaneous 
Dimensions240 x 280 x 220 mm (W x D x H)
including injectors
Weight   4 kg
OSWin XP, Win Vista, Win 7
Regulations   CE
Operation Temperature15 – 35 °C

 

Accessories

ICE Software Upgrades

Advanced functionality can be added to the ICE software by upgrading to the Advanced version, e.g.

  •      extended reports for standard curve assays
  •      additional curve fit algorithms
  •      use of master and reference curves
  •      cut-off assay data reduction

Lumi Vials

Polystyrene vials are available in various sizes. The 3 mL vials are gamma irradiated to provide sterile conditions for cell cultures or the use of primary cells.

  •      Lumi vials 5 mL, 12 x 75 mm
  •      Lumi vials 3.5 mL, 12 x 55 mm
  •      Lumi vials 3 mL, 12 x 47 mm, irradiated

Test Devices & QC Services

For continuous verification of the instrument performance test tube can be applied by the user and Performance Qualification services done by experienced Berthold Technologies personnel are available.

Cleanit Daily Injector Cleaning Solution

For proper maintenance of the injection system the Cleanit Daily solution is recommended for regular cleaning. Daily cleaning ensures that accuracy and precision as well as a long life time of the injectors will be maintained.

Applications

Reporter gene assays

Dual Luciferase Reporter Gene Assay: Goloudina et al. (2012). Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. PNAS 109 (2) 68-75 Read more

ATP determination

ATP assay in platelets: Mushtaq et al. (2001). Critical Role for CD38-mediated Ca2+ Signaling in Thrombin-induced Procoagulant Activity of Mouse Platelets and Hemostasis. The Journal of Biological Chemistry 286, 12952-12958 Read more

Reactive Oxygen Species (ROS)

Reactive Oxygen Species in Tobacco seedlings: Dokládal et al. (2012). Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance. Journal of Experimental Botany 63 (5), 2203-2215 Read more

H202 chemiluminescence in Arabidopsis: Dubreuil-Maurizi et al. (2011). Glutathione Deficiency of the Arabidopsis Mutant pad2-1 Affects Oxidative Stress-Related Events, Defense Gene Expression, and the Hypersensitive Response. Plant Physiology 157 (4), 2000-2012 Read more

Calcium monitoring

Aequorine Assay in Yeast Cells: Csutora et al. (2005). Inhibition of phosphoglucomutase activity by lithium alters cellular calcium homeostasis and signaling in Saccharomyces cerevisiae. AJP Cellular Physiology 289, 58-67 Read more


Further applications
 

Luminescent bacteria - Water toxicity - Enzyme activity - Luminescent immuno assay (LIA) - Hygiene monitoring - Cell viability - DNA probe assay

Download
Brochure
Format Size
Bioanalytic Instruments - Product Portfolio Brief overview scope of supply Bioanalytic (June 2014) de, en PDF 693.31 KB
LB 9508 Lumat3 Brochure 2011 de, en PDF 1.08 MB
Order Information Consumables & QC Test Devices Version Jan. 2017 de, en PDF 153.63 KB

News about this product

Renowned for its sensitivity and ease-of-use the luminometer has been extended in its range of usability by adding the DualGlo setup for luciferase reporter gene assays with manual reagent addition.

Read more
28.09.2015