In vivo imaging is the non-invasive visualization of living organisms for research or diagnostic purposes. Generally speaking, this method can be divided in two key areas: anatomical/morphological imaging and molecular imaging. In molecular imaging cellular function or molecular processes are visualized, normally using biomarkers. While in anatomical imaging no marker is used and visualization is based on the intrinsic properties of the tissues and organs being observed, such as the attenuation of X-rays in the case of computed tomography. Molecular imaging very often uses biomarkers labeled with bioluminescence or fluorescence.
By using in vivo molecular imaging, multiple molecular events can be monitored simultaneously, for example, to visualize drug effects, to optimize drug and gene therapy, to monitor tumor growth or to study disease progression in both, living animals and plants. By providing insights into transcription–translation feedback loops, which are entrained by environmental signals such as light or temperature, even circadian clocks in plants can be monitored. Compared to other methods of measuring molecular processes, in vivo imaging has several important advantages:
- It provides the spatial location of the process or molecule.
- It is dynamic: changes in time can be followed.
- It can be repeated several times in the same individual.
- It allows the observation of a (mostly) undisturbed process: that means, results are physiologically relevant.
These advantages make in vivo imaging a very useful technology in many research and diagnostic fields.
In Vivo Imaging Application Notes
Measurement of Biophoton Emission with NightShade Measurement of Biophoton Emission in Plants – An Alternative Monitoring System for Stress Factors
PDF | 1.4 MB
Circadian Clock with NightSHADE Getting to Know The Circadian Clock and Plant Growth With NightSHADE
PDF | 170.2 KB
Measurement of delayed fluorescence with NightShade Measurement of Delayed Fluorescence in Plants - a Monitoring System for Stress Factors
PDF | 560.7 KB
Fluorescence in different subcellular localizations with NightShade Detection of Fluorescence in Transgenic Arabidopsis Lines Expressing Fluorophores in Different Subcellular Localizations
PDF | 840.6 KB
Analysis of circadian rhythms using NightShade Improved experimental setup for analysis of circadian rhythms using the NightShade Plant In Vivo Imaging System
PDF | 479.1 KB
Trans-illumination in GFP-Expressing Plants using NightShade Trans-illumination – a Solution for Excitation of GFP Expressing Plants in Petri Dishes for In Vivo Imaging using the NightShade Plant In Vivo Imaging System
PDF | 484.8 KB
Fluorescent nanoparticle imaging using NightOWL Fluorescence in Vivo Imaging of labeled nanoparticles in tumour-bearing mice with NightOWL II In Vivo Imaging System
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Bioluminescence Imaging using NightOWL Bioluminescence Imaging using NightOWL LB 981 NC 100. Cells expressing luciferase gene under the control of a constitutive promoter were used as a model of in vivo proliferation of cancer cells.
PDF | 307.0 KB
Luciferin bioavailability in mice during in-vivo imaging To determine the optimal luciferase activity detection time, time course experiments were performed.
PDF | 315.7 KB
Monitoring of Renilla Luciferase Activities in-vitro and in-vivo The expression of a novel EnduRen™ and ViviRen™ Renilla luciferase reporter gene (Promega) in vitro and in vivo is explored
PDF | 385.0 KB