The Orion II LB 965 meets all currently known requirements for an all-purpose microplate luminometer. The reader supports all basic luminescence technologies including:

Highlights

High performance luminescence

The outstanding sensitivity of Orion II is the result of the unique design, advanced engineering, and high-quality workmanship. All functions of the Orion II are seamlessly integrated to render superior, user-friendly performance.

The detector is operated in photon counting mode, which guarantees the lowest signal background for excellent signal to noise ratio and the highest linearity. The plate adjustment mechanism automatically compensates for variations in microplate size. The built-in safety circuit protects the detector from potential damage resulting from accidental exposure to high levels of light. In addition, a warning is produced should a sample ever exceed measurement range.

 

Advanced shaking and temperature control options

The Orion II is more than a basic luminometer. For assays that require shaking, three mixing modes are available: linear, orbital and cross shaking. Flexible speed and amplitude settings allow easy adaptation to any assay protocol. Orion II offers two different types of sample incubation: top and bottom microplate incubation ensures exceptional temperature stability in the sample chamber for temperature control up to 50°C. For applications that require up to 42°C incubation only, the optional bottom heating function is recommended.

Powerful crosstalk control – reliable results

Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples?  Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The special design of the Orion II does almost eliminate this problem: with a crosstalk of 3 x 10-5, you never have to worry about the position of your high-intensity samples in your plate.

Up to 4 injectors with low dead volume and flexible positioning

The combination of reagent addition options is virtually unlimited. Up to four automatic reagent injectors are available with injection volumes of 10 to 150 μL each. For increased throughput, up to 2 injectors can be installed in measurement position for simultaneous injection. The optimized reagent holders accommodate any shape and any size bottle in a position that allows the complete use of valuable reagent.

Easy waste management

Thanks to its built-in waste pump, you don’t need to worry about loading microplates, waste containers or removal of injector tips for washing and filling the reagent lines. The built-in waste pump takes care of the safe removal of excess liquids.

 

Optimized injection for faster throughput

The Orion II system has the capability to run samples and process measurements in the most time efficient manner. The proprietary Double Injection Design (DID) allows parallel processing of samples saving valuable assay time. DID has been applied to both 96 well and 384 well configurations.