Optimized settings for protein kinase activity screening using the Tristar 5 Multimode Reader and the Transcreener ® ADP2 TR-FRET Red assay
Protein kinases are important targets for the development of new drugs. Its dysfunctional activity has been implicated in many diseases, ranging from cancer to inflammation and immune disorders. In order to study the regulation of protein kinases, it is of the utmost importance to be able to measure the activity of the kinases of interest.
The Transcreener® ADP2 TR-FRET Red Assay is a highly selective immunoassay for the measurement of kinase activity. It has been optimized for efficient high-throughput screening (HTS) using a convenient mix-and-read format. Its time-gated detection method and far-red fluorescence largely eliminate interference that can result from test compounds and light scattering. Learn how to measure kinase activity with high performance using the Transcreener® ADP2 TR-FRET Red Assay and the Tristar 5 Multimode Reader.
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