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Detection Modes

Detection ModesTristar 3Apollo 11CentroCentro XS³Orion II
Absorbance (Vis)     
Absorbance (UV)     
Fluorescence (Vis)     
Fluorescence (UV)     
Glow Luminescence     
Flash Luminescence     

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Technical Features

Technical FeaturesTristar 3Tristar 5Apollo 11CentroCentro XS³Orion II
Bottom Reading      
Monochromator for absorbance and excitation      
Monochromator for emission      
Injectors   up to 3up to 3up to 4
Temperature control      
Gas connection      
Absorbance spectral scanning      
Fluorescence spectral scanning      
Luminescence spectral scanning      
96 well      
384 well      
6-384 well      


Assay Types

Assay TypesTristar 3Tristar 5Apollo 11CentroCentro XS³Orion II
Endpoint assays      
Kinetic assays      
Well scanning      
Fluorescence immunoassays      
Luminescence immunoassays      


Protein Concentration

Protein ConcentrationTristar 3Tristar 5Apollo 11CentroCentro XS³Orion II
Bradford, BCA, Lowry      
Protein (A280)      
Low volume absorbance (2 µL)      
NanoOrange®, CBQCA, Quant-iT™      


Nucleic Acid Concentration

Nucleic Acid ConcentrationTristar 3Tristar 5Apollo 11CentroCentro XS³Orion II
DNA, RNA, oligos (A260)      
Low volume DNA samples (2 µL)      

RiboGreen®, PicoGreen®, OliGreen®

Quant-iT™ RNA      


Reporter Genes

Reporter GenesTristar 3Tristar 5Apollo 11CentroCentro XS³Orion II
Secreted Alkaline Phosphatase (SEAP)      
ß-Galactosidase (ß-Gal)      
ß-Glucuronidase (GUS)      
Dual Luciferase      
GFP, CFP, YFP      


Cell Viability, Proliferation and Death

Cell Viability, Proliferation and DeathTristar 3Tristar 5Apollo 11CentroCentro XS³Orion II

MTT, XTT, Crystal Violet, SRB

Fluorescent Live/Dead kits, Alamar Blue      
Calcein AM, CyQUANT®      

SYTOX® Green, ethidium homodimer



Enzyme Assays

Enzyme AssaysTristar 3Tristar 5Apollo 11CentroCentro XS³Orion II
ATP (luciferase)      
NADH (dehydrogenases)      
Phosphatases/Kinases activity      
Protease activity      


HTS Assays

HTS AssaysTristar 3Apollo 11CentroCentro XS³Orion II

ABEL® Luminescent antioxidant test (Knight Scientific)

Adapta™ (Invitrogen)     

AlphaScreen® (Perkin Elmer)

ATP bioluminescence     
DELFIA® (Perkin Elmer)     
DLR™ (Promega)     
Dual-Glo™ (Promega)     
GeneBLAzer™ (Invitrogen)     
HitHunter® (DiscoveRx, luminescence)     

HitHunter® (DiscoveRx, FP)

HTRF® (Cisbio)     

Intracellular calcium (Ca2+ assay)

Ion Channel - VSP (Invitrogen)     
LanthaScreen® (Invitrogen)     
MycoAlert™ (Cambrex)     
P450-Glo™ (Promega)     
PKLight® (Cambrex)     
PolarScreen™ (Invitrogen)     
Predictor™ (Invitrogen)     
Transcreener® ADP² FP (BellBrook Labs)     
Z'-Lyte (Invitrogen)     

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ABEL is registered trademark of Knight Scientific Limited.

Chroma-Glo, Kinase-Glo, DLR, Dual-Glo and P450-Glo are trademarks of Promega Corp.

MycoAlert and PK Light are trademarks of Cambrex.

HitHunter is a trademark of DiscoveRx.

Adapta, GeneBLAzer, Predictor, NanoOrange, PicoGreen, RiboGreen, OliGreen, Quant-iT, CBQCA, PolarScreen, LanthaScreen, Ion Channel VSP, Z'-Lyte, Alamar Blue, and Alexa Fluor are trademarks or registered trademarks of Thermo Fisher Scientific.

AlphaScreen, AlphaLISA, AlphaPlex, DELFIA, and LANCE are registered trademarks of Perkin Elmer Inc.

HTRF is a registered trademark of Cisbio International.

Transcreener is a patented technology of BellBrook Labs.

Cy Dyes are trademarks of GE Healthcare.