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Detection Modes

Detection ModesTristar 3ApolloCentroCentro XS
Absorbance (Vis)    
Absorbance (UV) from 340 nm  
Fluorescence (Vis)    
Fluorescence (UV)    
FRET    
FP    
TRF    
HTRF®    
AlphaScreen®    
Glow Luminescence    
Flash Luminescence    
BRET    

Technical Features

Technical FeaturesTristar 3ApolloCentroCentro XS
Bottom Reading    
Monochromator    
Injectors  up to 3up to 3
Temperature control    
Shaking    
Gas connection    
Absorbance spectral scanning    
Fluorescence spectral scanning    
Luminescence spectral scanning    
96 well    
384 well    
6-48 well    
1536 well    

 

Assay Types

Assay TypesTristar 3ApolloCentroCentro XS
Endpoint assays    
Kinetic assays    
Well scanning    
Immunoassays    
ELISA    
Fluorescence immunoassays    
Luminescence immunoassays    

 

Protein Concentration

Protein ConcentrationTristar 3ApolloCentroCentro XS
Bradford, BCA, Lowry    
Protein (A280)    
Low volume absorbance (2 µL)    
NanoOrange®, CBQCA, Quant-iT™    

 

Nucleic Acid Concentration

Nucleic Acid ConcentrationTristar 3ApolloCentroCentro XS
DNA, RNA, oligos (A260)    
A260/A280    
Low volume DNA samples (2 µL)    

RiboGreen®, PicoGreen®, OliGreen®

    
Quant-iT™ RNA    

 

Reporter Genes

Reporter GenesTristar 3ApolloCentroCentro XS
Secreted Alkaline Phosphatase (SEAP)    
ß-Galactosidase (ß-Gal)    
ß-Glucuronidase (GUS)    
Luciferase    
Dual Luciferase    
GFP, CFP, YFP    

 

Cell Viability, Proliferation and Death

Cell Viability, Proliferation and DeathTristar 3ApolloCentroCentro XS

MTT, XTT, Crystal Violet, SRB

    
Fluorescent Live/Dead kits, Alamar Blue    
Calcein AM, CyQUANT®    

SYTOX® Green, ethidium homodimer

    

 

Enzyme Assays

Enzyme AssaysTristar 3ApolloCentroCentro XS
ATP (luciferase)    
NADH (dehydrogenases)    
Phosphatases/Kinases activity    
Protease activity    

 

HTS Assays

HTS AssaysTristar 3ApolloCentroCentro XS

ABEL® Luminescent antioxidant test (Knight Scientific)

    
Adapta™ (Invitrogen)    

AlphaScreen® (Perkin Elmer)

    
ATP bioluminescence    
DELFIA® (Perkin Elmer)    
DLR™ (Promega)    
Dual-Glo™ (Promega)    
GeneBLAzer™ (Invitrogen)    
HitHunter® (DiscoveRx, luminescence)    

HitHunter® (DiscoveRx, FP)

    
HTRF® (Cisbio)    

Intracellular calcium (Ca2+ assay)

    
Ion Channel - VSP (Invitrogen)    
LanthaScreen® (Invitrogen)    
MycoAlert™ (Cambrex)    
P450-Glo™ (Promega)    
PKLight® (Cambrex)    
PolarScreen™ (Invitrogen)    
Predictor™ (Invitrogen)    
Transcreener® ADP² FP (BellBrook Labs)    
Z'-Lyte (Invitrogen)    

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ABEL is registered trademark of Knight Scientific Limited.

Chroma-Glo, Kinase-Glo, DLR, Dual-Glo and P450-Glo are trademarks of Promega Corp.

MycoAlert and PK Light are trademarks of Cambrex.

HitHunter is a trademark of DiscoveRx.

Adapta, GeneBLAzer, Predictor, NanoOrange, PicoGreen, RiboGreen, OliGreen, Quant-iT, CBQCA, PolarScreen, LanthaScreen, Ion Channel VSP, Z'-Lyte, Alamar Blue, and Alexa Fluor are trademarks or registered trademarks of Thermo Fisher Scientific.

AlphaScreen, AlphaLISA, AlphaPlex, DELFIA, and LANCE are registered trademarks of Perkin Elmer Inc.

HTRF is a registered trademark of Cisbio International.

Transcreener is a patented technology of BellBrook Labs.

Cy Dyes are trademarks of GE Healthcare.