The accurate, RNA and proteins, and the assessment of its purity, are of highest importance for all kinds of downstream applications. The applications are qRT PCR, sequencing, microarray, western blotting and many others. Too often the available quantity of biological samples is often very limited, especially lysates of cells grown in microplates and small tissue samples from patients or laboratory animals. This is why a UV-Vis spectrophotometer requiring the smallest sample volume is very valuable in laboratories using Molecular Biology methods. A highly reproducible instrument especially at low concentrations with a large linear range are also very desirable.
Our microvolume spectrophotometers have been optimized for the
In addition to the default quantification settings for dsDNA, RNA and ssDNA, the software of our microvolume spectrophotometers enables to use custom extinction coefficients. This enables the user to measure other sample types, such as siRNA or oligonucleotides.
Since we are able to measure absorption not only in the UV but also in the visible range, our microvolume spectrophotometers can also be used to
A wide range of other dyes can also be measured, e.g. for microarray applications, protein markings or any colorimetric method with limited sample availability. The Colibri's versatility makes it a great addition to any life sciences laboratory.
Is UV-Vis absorbance not sensitive enough for your needs? Or maybe you need a solution for larger numbers of samples? Then you may be interested on measuring either absorbance or fluorescence in our, or to learn more about solutions.