DNA quantification methods

The most popular DNA quantification methods are based on UV-Vis- or fluorescence spectroscopy. Both methods, as well as other procedures, have advantages and disadvantages, which is why it is important to consider the methods in the context of the entire analysis, including upstream and downstream processes.

Instruments for DNA quantification

For DNA quantification, a wide range of instruments are suitable, from small microvolume spectrophotometers to complex multimode microplate readers. Therefore, the equipment is also an important point to consider when choosing a method for DNA quantification. Find out what options are available, what methods they are suitable for and what accessories and consumables are recommended.

Influence of DNA purity on quantification

Contaminations in the purified DNA can lead to unreliable quantification and to problems in downstream assays. Learn more about how to assess the purity of your DNA and to eliminate inaccuracies caused by contaminants.

Application Notes related to DNA quantification

Measurement of ssDNA using the Colibri Low volume measurement of ssDNA using the Colibri Microvolume Spectrophotometer for DNA quantification

PDF | 92.3 KB

RNA quantification with the Colibri Low volume measurement of RNA using the Colibri Microvolume Spectrophotometer

PDF | 170.5 KB