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Detection Modes

Detection ModesTristar 3Tristar 5Apollo 11CentroPROCentroCentro XS³Orion IITristar²Tristar² SMithrasMithras²
Absorbance (Vis)           
Absorbance (UV)           
Fluorescence (Vis)           
Fluorescence (UV)           
FRET           
FP           
TRF           
HTRF®*          
AlphaScreen®           
Glow Luminescence           
Flash Luminescence           
BRET           

* Please inquire

Technical Features

Technical FeaturesTristar 3Tristar 5Apollo 11CentroPROCentroCentro XS³Orion IITristar²Tristar² SMithrasMithras²
Bottom Reading           
Monochromator for absorbance and excitation           
Monochromator for emission           
Injectors    up to 3up to 3up to 4up to 3up to 3up to 4up to 4
Temperature control           
Shaking           
Gas connection           
Absorbance spectral scanning           
Fluorescence spectral scanning           
Luminescence spectral scanning           
96 well           
384 well           
6-384 well           
6-1536           

 

Assay Types

Assay TypesTristar 3Tristar 5Apollo 11CentroPROCentroCentro XS³Orion IITristar²Tristar² SMithrasMithras²
Endpoint assays           
Kinetic assays           
Well scanning           
Immunoassays           
ELISA           
Fluorescence immunoassays           
Luminescence immunoassays           

 

Protein Concentration

Protein ConcentrationTristar 3Tristar 5Apollo 11CentroPROCentroCentro XS³Orion IITristar²Tristar² SMithrasMithras²
Bradford, BCA, Lowry           
Protein (A280)           
Low volume absorbance (2 µL)           
NanoOrange®, CBQCA, Quant-iT™           

 

Nucleic Acid Concentration

Nucleic Acid ConcentrationTristar 3Tristar 5Apollo 11CentroPROCentroCentro XS³Orion IITristar²Tristar² SMithrasMithras²
DNA, RNA, oligos (A260)           
A260/A280           
Low volume DNA samples (2 µL)           

RiboGreen®, PicoGreen®, OliGreen®

           
Quant-iT™ RNA           

 

Reporter Genes

Reporter GenesTristar 3Tristar 5Apollo 11CentroPROCentroCentro XS³Orion IITristar²Tristar² SMithrasMithras²
Secreted Alkaline Phosphatase (SEAP)           
ß-Galactosidase (ß-Gal)           
ß-Glucuronidase (GUS)           
Luciferase           
Dual Luciferase           
GFP, CFP, YFP           

 

Cell Viability, Proliferation and Death

Cell Viability, Proliferation and DeathTristar 3Tristar 5Apollo 11CentroPROCentroCentro XS³Orion IITristar²Tristar² SMithrasMithras²

MTT, XTT, Crystal Violet, SRB

           
Fluorescent Live/Dead kits, Alamar Blue           
Calcein AM, CyQUANT®           

SYTOX® Green, ethidium homodimer

           

 

Enzyme Assays

Enzyme AssaysTristar 3Tristar 5Apollo 11CentroPROCentroCentro XS³Orion IITristar²Tristar² SMithrasMithras²
ATP (luciferase)           
NADH (dehydrogenases)           
Phosphatases/Kinases activity           
Protease activity           

 

HTS Assays

HTS AssaysTristar 3Tristar 5Apollo 11CentroPROCentroCentro XS³Orion IITristar²Tristar² SMithrasMithras²

ABEL® Luminescent antioxidant test (Knight Scientific)

           
Adapta™ (Invitrogen)*          

AlphaScreen® (Perkin Elmer)

           
ATP bioluminescence           
DELFIA® (Perkin Elmer)*          
DLR™ (Promega)           
Dual-Glo™ (Promega)           
GeneBLAzer™ (Invitrogen)           
HitHunter® (DiscoveRx, luminescence)           

HitHunter® (DiscoveRx, FP)

 *      *  
HTRF® (Cisbio)*          

Intracellular calcium (Ca2+ assay)

           
Ion Channel - VSP (Invitrogen)           
LanthaScreen® (Invitrogen)*          
MycoAlert™ (Cambrex)           
P450-Glo™ (Promega)           
PKLight® (Cambrex)           
PolarScreen™ (Invitrogen) *      *  
Predictor™ (Invitrogen)           
Transcreener® ADP² FP (BellBrook Labs) *      *  
Z'-Lyte (Invitrogen)           

* Not enought data. Please inquire.

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ABEL is registered trademark of Knight Scientific Limited.

Chroma-Glo, Kinase-Glo, DLR, Dual-Glo and P450-Glo are trademarks of Promega Corp.

MycoAlert and PK Light are trademarks of Cambrex.

HitHunter is a trademark of DiscoveRx.

Adapta, GeneBLAzer, Predictor, NanoOrange, PicoGreen, RiboGreen, OliGreen, Quant-iT, CBQCA, PolarScreen, LanthaScreen, Ion Channel VSP, Z'-Lyte, Alamar Blue, and Alexa Fluor are trademarks or registered trademarks of Thermo Fisher Scientific.

AlphaScreen, AlphaLISA, AlphaPlex, DELFIA, and LANCE are registered trademarks of Perkin Elmer Inc.

HTRF is a registered trademark of Cisbio International.

Transcreener is a patented technology of BellBrook Labs.

Cy Dyes are trademarks of GE Healthcare.