Sirius 2 LB 9526 Brochure
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The Sirius 2 detects less than 0.5 zmol of firefly luciferase (1 amol ATP/tube). Light collection is maximized through the close proximity of the sample to the detector and the use of optimized light reflectors surrounding the sample vial. The detector is operated in photon counting mode, which ensures a very low background signal for excellent signal to noise ratio and the highest linearity.
The sample chamber is designed for performance and flexibility: from 35 mm petri dishes to microfuge format to 20 mL vials, just pick the sample format that fits your application best. The optional sample holders enable accurate injection into both, microcentrifuge tubes and standard luminescence tubes.
The system is compatible with a variety of sample formats:
The touchscreen-driven software is very intuitive and operated by touch gestures. The easy-to-use software provides advanced features, such as handling of replicates, dual assay calculations, and display of graphics.
Data can be downloaded easily onto a USB stick or printed. The results can be exported for further calculation in Excel or other programs.
Closing of the sample drawer automatically initiates the measurement. There´s no need to press any key. The automatic light shutter protects the detector from ambient light. This makes the system ready for measurement from the moment the door is closed. This means that there is no need to set a long delay before the measurement. This is necessary with most other tube luminometers so that the background can return to the initial level after the door has been opened.
The system is available with up to two syringe-type automatic reagent injectors. The injection volume range is 20–500 μL. This very broad volume range allows the Sirius 2 to adapt to any assay using injectors. Ready-to go sample holders for precise injection are available for 1.5 ml and 2 ml microfuge tubes, as well as for 55 mm and 75 mm, 12 mm diameter tubes (standard format for most luminescence assays).
Low-noise photomultiplier tube in single photon counting mode
|Measurement modes||Endpoint, Kinetics, Multiple Kinetics|
|Spectral range luminescence||300-600 nm|
|Injectors||Up to 2 syringe injectors|
Luminescence tubes, 12 x 55 mm
Luminescence tubes, 12 x 75 mm
Microcentrifuge tubes, 1.5 and 2 mL
Scintillation vials (up to 20 mL)35 mm Petri dishes
|Luminescence sensitivity||<1 amol ATP/tube (ATP HS assay)|
|Dynamic range||6 orders of magnitude|
|Display (integrated computer)||7” capacitive touch, gesture control, 480 x 800 pixels|
|External interfaces (integrated computer)||2x USB 2.0, 1x Ethernet (RJ-45)|
|Network or USB printing (PCL6 or Postscript 3)|
|Data export||CSV (Excel), PDF, HTML formats|
|Available protocols|| |
|Power Supply||110-240 V, 24 V/2.5 A DC|
|Temperature tolerance||Storage: 0-40° C|
Operation: 15-35° C
Storage: up to 80% non-condensingOperation: up to 70% non-condensing
Dimensions (W x D x H)
|349 x 150 x 241 mm|
ATP detection limit with Sirius L Below amol ATP detection limit with Sirius L Tube Luminometer and ATP Reagent SS from BioThema
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Berthold Technologies GmbH & Co.KG
Calmbacher Str. 22
75323 Bad Wildbad
Tel.: (+49) 7081 177 0
Fax: (+49) 7081 177 100