ABEL® Cell Activation Assay for Whole Blood or Isolated Cells with Pholasin® and…
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The Sirius 2 detects less than 0.5 zmol of firefly luciferase (1 amol ATP/tube). Light collection is maximized through the close proximity of the sample to the detector and the use of optimized light reflectors surrounding the sample vial. The detector is operated in photon counting mode, which ensures a very low background signal for excellent signal to noise ratio and the highest linearity.
The sample chamber is designed for performance and flexibility: just pick the sample format that fits your application best. The optional sample holders enable accurate injection into both, microcentrifuge tubes and standard luminescence tubes.
The system is compatible with a variety of sample formats:
The touchscreen-driven software is very intuitive and operated by touch gestures. The easy-to-use software provides advanced features, such as handling of replicates, dual assay calculations, and display of graphics.
Data can be downloaded easily onto a USB stick or printed. The results can be exported for further calculation in Excel or other programs.
Closing of the sample drawer automatically initiates the measurement. There´s no need to press any key. The automatic light shutter protects the detector from ambient light. This makes the system ready for measurement from the moment the door is closed. This means that there is no need to set a long delay before the measurement. This is necessary with most other tube luminometers so that the background can return to the initial level after the door has been opened.
The system is available with up to two syringe-type automatic reagent injectors. The injection volume range is 20–500 μL. This very broad volume range allows the Sirius 2 to adapt to any assay using injectors. Ready-to go sample holders for precise injection are available for 1.5 ml and 2 ml microfuge tubes, as well as for 55 mm and 75 mm, 12 mm diameter tubes (standard format for most luminescence assays).
Low-noise photomultiplier tube in single photon counting mode
|Measurement modes||Endpoint, Kinetics, Multiple Kinetics|
|Spectral range luminescence||300-600 nm|
|Injectors||Up to 2 syringe injectors|
Luminescence tubes, 12 x 55 mm
Luminescence tubes, 12 x 75 mm
Microcentrifuge tubes, 1.5 and 2 mL
|Luminescence sensitivity||<1 amol ATP/tube (ATP HS assay)|
|Dynamic range||6 orders of magnitude|
|Display (integrated computer)||7” capacitive touch, gesture control, 480 x 800 pixels|
|External interfaces (integrated computer)||2x USB 2.0, 1x Ethernet (RJ-45)|
|Network or USB printing (PCL6 or Postscript 3)|
|Data export||CSV (Excel), PDF, HTML formats|
|Available protocols|| |
|Power Supply||110-240 V, 24 V/2.5 A DC|
|Temperature tolerance||Storage: 0-40° C|
Operation: 15-35° C
Storage: up to 80% non-condensingOperation: up to 70% non-condensing
Dimensions (W x D x H)
|349 x 150 x 241 mm|
The Sirius 2 is a very recent instrument and thus not many papers using it have yet been published. Here you have a selection of articles which feature the Sirius 2 or the Sirius L, which are very similar.
Xia Guo, Christudas Sunil, Oluwaseun Adeyanju, Andrew Parker, Steven Huang, Mitsuo Ikebe, Torry A. Tucker, Steven Idell, and Guoqing Qian
Firefly luciferase reporter
Luigi D’Angelo, Elisa Astro, Monica De Luise, Ivana Kurelac, Nikkitha Umesh-Ganesh, Shujing Ding, Ian M. Fearnley, Giuseppe Gasparre, Massimo Zeviani, Anna Maria Porcelli, Erika Fernandez-Vizarra, and Luisa Iommarini
ATP synthesis rate assay
Simona Plesselova, Pablo Garcia-Cerezo, Victor Blanco, Francisco J. Reche-Perez, Fernando Hernandez-Mateo, Francisco Santoyo-Gonzalez, María Dolores Giron-Gonzalez, and Rafael Salto-Gonzalez
Journal of Medicinal Chemistry
Fahimeh Ghavamipour, Hossein Rahmani, Maryam Shanehsaz, Khosro Khajeh, Manouchehr Mirshahi, and Reza H. Sajedi
Journal of Nanobiotechnology
Yuwan Zhao, Xin Zeng, Huancheng Tang, Dongcai Ye and Jianjun Liu
Caspase-Glo® 3/7, ATP Assay
Mingning Qiu, Sai Zhang, Longzhi Ke, Huancheng Tang, Xin Zeng, and Jianjun Liu
ATP Assay, Caspase-Glo® 3/7, Caspase-Glo® 9
Gabriela Jírová, Alena Vlková, Martina Wittlerová, Markéta Dvořáková, Lucie Kašparová, Jan Chrz, Kristina Kejlová, Zdeňka Wittlingerová, Magdaléna Zimová, Barbora Hošíková, Jana Jiravová, Hana Kolářová
Water toxicity with Vibrio fischeri
Zinaida M. Kaskova, Felipe A. Dörr, Valentin N. Petushkov, Konstantin V. Purtov, Aleksandra S. Tsarkova, Natalja S. Rodionova, Konstantin S. Mineev, Elena B. Guglya, Alexey Kotlobay, Nadezhda S. Baleeva, Mikhail S. Baranov, Alexander S. Arseniev, Josef I. Gitelson, Sergey Lukyanov, Yoshiki Suzuki, Shusei Kanie, Ernani Pinto, Paolo Di Mascio, Hans E. Waldenmaier, Tatiana A. Pereira, Rodrigo P. Carvalho, Anderson G. Oliveira, Yuichi Oba, Erick L. Bastos, Cassius V. Stevani, and Ilia V. Yampolsky
Ignacio A. Rodriguez-Brenes, Andrew Hofacre, Hung Fan and Dominik Wodarz
PLoS Computational Biology
Mingning Qiu, Lieqian Chen, Guobin Tan, Longzhi Ke, Sai Zhang, Hege Chen and Jianjun Liub
Caspase-Glo® 3/7, ATP Assay
Juan‐Ping Yu, Xi‐Guang Xu, Rui‐Juan Ma, Shi‐Ni Qin, Cong‐Rong Wang, Xiao‐Bo Wang, Ming Li, Ming‐Song Li, Qiang Ma, and Wei‐Wen Xu
Journal of Clinical Laboratory Analysis
You can find additional publications which have used older Sirius instruments in publication databases.
Please keep in mind that the list provided is not exhaustive, but it offers valuable examples of experiments performed with our instruments and published in peer-reviewed journals.