Compared to traditional dioxetanes, AquaSpark™ probes have unique properties:

  • They can work as single agents, without the need for enhancers, co-substrates or any additional reagent.
  • They have a higher efficiency and sensitivity over currently existing probes: an increase in chemiluminescent intensity of up to 3000 times can be observed in some cases.
  • They are especially designed to work under physiological conditions and can be used ex-vivo and in-vivo.

Several AquaSpark™-based products are already available for research and diagnostic applications, including substrates of alkaline phosphatase, alpha-glucosidase and beta-galactosidase to be used in chemiluminescent immunoassays (CLIA), AquaSpark-caprylate to test for Salmonella and Pseudomonas strains, and peroxide probes for detection of any H2O2-releasing oxidase activity, among others.

AquaSpark™ probes are promising in many other applications, such as chemiluminescent microscopy and chemiluminescent in vivo imaging.

Recommended instruments for AquaSpark™