AlphaScreen® relies on hydrogel coated Donor and Acceptor beads providing functional groups for conjugation to biomolecules.

AquaSpark™ is a new generation of chemiluminescent probes based on dioxetane derivatives that exhibit enhanced properties over traditional dioxetanes.

ATP measurements are used to monitor substances and surfaces for bacterial contamination, as well as for waste water analysis.

BRET (Bioluminescence Resonance Energy Transfer) is based on the fact that the energy derived from a luciferase reaction can be used to excite a fluorescent protein if the latter is in close proximity to the luciferase enzyme.

CALUX® Assays can be used to detect dioxins, dioxin-like PCB's, (anti)estrogens and (anti)androgen compounds, and other chemicals, by using a luminometer.

The activity of caspases can be monitored using luminescence, fluorescence intensity or TRF, and is a key method in apoptosis research.

Cellular chemiluminescence (CL) is the production of light by neutrophil granulocytes, monocytes or macrophages when phagocytosis is activated.

Coating means the immobilization of antigen, antibodies or any other compound on the well surface, for the purpose of a binding assay. The „coated plate“ is part of a commercial ELISA kit, or of a home-brew (self-made) ELISA assay.

Fluorescence polarisation (FP) is the ideal tool to measure the binding of a small molecule to a much larger one.

FRET (Fluorescence Resonance Energy Transfer) can be performed in a microplate reader to detect the interactions of proteins and nucleic acids.

HTRF combines time-resolved fluorescence with classical FRET to create assays of high robustness and sensitivity, which can be measured in a microplate reader.

ATP is present in all living cells and is therefore an indicator of biological contamination. Learn more about using ATP quantification for hygiene monitoring.

Immunoassays offer the specific and sensitive detection of analytes of interest thanks to the specificity of the antibody-antigen reaction.

Intracellular Calcium is important for the study of ion channels and G protein-coupled receptors. Learn how to measure it using luminescence or fluorescence.

Kinases modify the activity of specific proteins and are often determined using assays based on luminescence or Time-Resolved Fluorescence (TRF).

A luminometer is an instrument that measures light coming from a sample. Learn more about luminometers and find the right luminometer for your needs here.

A monochromator is one of the possible means of wavelength selection in microplate readers, spectrophotometers and other measurement instruments. Learn more about monochromators here.

Quantification of protein concentration in solution has since long been of essential importance in biological laboratories.

Reporter genes are an invaluable tool in gene expression studies. Luciferase assays are the most popular ones, but there are other possibilities.

Luminescence measurements are expressed as RLU (Relative Light Unit), but what does that mean? Relative to what? Learn more about luminescence measurements.

SPARCL is a proximity-dependent chemiluminescent technology for the detection of specific binding interaction between two binding partners.

Time-Resolved FRET combines time-resolved methodologies with classical FRET to create assays of high robustness and sensitivity.

The term Time-Resolved Fluorescence in the field of Life Sciences can be sometimes confusing, as it can be used with different meanings.

The use of intact luminous photobacteria or dinoflagellates for toxicity assessment of water samples has many clear advantages.

AlphaScreen® is a registered trademark of PerkinElmer.

HTRF® is a registered trademark of Cisbio Bioassays.

SPARCL® is a registered trademark of Lumigen, Inc.