Brochure of the Zoom HT LB 920 Microplate Washer
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It takes only 17 seconds for a triple wash of a 96 well microplate. This, combined with the unique one-rail plate transfer design that moves the plate short distance from the stack to the wash position, enables a typical throughput of 150 washed plates per hour, or up to 1050 plates per day (3 x 300 µL, 7 h per day, 1 single washer).
Each wash channel has its own aspirate tip and dispense tip, which allows simultaneous washing of all 96 wells. The same 96 channel wash head is used for 384 well washing in four quadrants, making it unnecessary to purchase different wash heads for different plate types or applications, and having to replace them. Rapid positioning of the wash head provides 384 well wash speeds comparable to 384 channel washers. Large diameter aspirate tips, 3-dimensional (3D) tip positioning and a constant flow rate vacuum pump ensure a smooth, efficient washing performance.
Two wash modes are available: Regular wash mode and Superwash mode (also known as “Overflow mode”). In the regular wash mode, the wash fluid dispensing and evacuation follow each other. In the Superwash mode, the wash fluid dispensing and evacuation are conducted simultaneously creating a continuous wash fluid flow through the well. The Superwash flow rate is adjustable. For additional wash procedure optimization, the Zoom HT supports creating and storing of the 3D tip positioning profile for each plate type and assay. This profile defines the aspirate tip horizontal and vertical positions thereby mapping how the well is aspirated. In addition, the speed with which the tip is immersed into the well is adjustable. The possibility of fine-tuning all parameters of the wash procedure allow to wash even loosely adherent cultured cells. With the Zoom HT, residual wash fluid volumes of less than 1 μL/well (in presence of surfactant in the wash fluid) can be achieved.
The discarded wash fluids are captured by a self-emptying waste trap, which allows uninterrupted processing of large plate batches. Biohazard waste management is supported by automatic addition of a decontamination solution. The decontaminated waste is continuously drained from the trap.
The Zoom HT is available in different versions with one, two or four wash buffer inlets. This function is advantageous for applications that require sequential cleaning with different buffers or for an automatic cleaning routine after using the system.
The unique design of the dispense manifold eliminates the need for special cleaning options such as ultrasonic cleaning. The aspirate head is maintenance free. The large aspirate tip diameter, combined with a continuous vacuum, reliably prevents an accumulation of reagent residue and clogging.
The optional Dispense Module is an easy-to-install plug-and-play device. It requires no calibration and is extremely easy to use. With the addition of a dispense module, the Zoom Washer becomes an efficient washer-dispenser combination system. The Dispensing Module provides timely and accurate single or dual-channel dispensing of reagents. Berthold Technologies uses a proprietary syringe-based liquid distribution system for reagent dispensing. Direct drive and positive displacement syringes ensure the highest precision and accuracy of dispensing. The dispense module feeds one or two 8- or 16-way manifolds (for 96 or 384 well microplates).
96 channel wash head for fast processing of 96 and 384 well microplates
|Wash modes||Standard and Superwash|
|Wash fluid selection||1, 2 or 4 inlet versions|
|Wash volume||5-300 µL|
|Aspirate tip descent speeds||Slow, Medium, Fast|
|Soak time||0-99 s|
|Shaking||Up to 80 s, amplitude 1 mm, frequency 12 Hz|
|Number of programs||Up to 99|
|Wash cycles per program||Up to 99|
|Program protection||Administrator mode to prevent unauthorized changes|
96-well plates and strips, 384-well plates; dimensions according to ANSI SLAS
One-rail design connecting wash, dispense and storage positions; plate capacity: 30 plates per tower
|Wash head dispense accuracy|
±2% typical, 50-300 µL
|Wash head dispense precision|
CV ≤2.5% @ 200 µL
CV ≤3% @ 100 µLCV ≤4.5% @ 50 µL
|Residual volume||<2 µL/well|
|Plate processing speed|
96-well plate, 300 µL (1 cycle), including stack: 14 s
96-well plate, 300 µL (3 cycles), including stack: 24 s384-well plate, 75 µL (3 cycles), including stack: 44 s
|Power supply||110-120 V 50/60 Hz, or 220-240 V, 50/60 Hz; 300 VA|
|Operating temperature||10-40° C|
|Liquid path materials|
Stainless steel, Teflon®, PVC, Norprene, Silicone, Polysulfone
|Dimensions (W x D x H)||690 x 560 x 610 mm|
8-way and 16-way manifolds available
|Dispense manifold ports||2 in direct proximity to wash head for immediate dispensing after aspiration|
|Syringe size||2 x 10 mL, separate channels|
|Dispensing technology||Positive displacement|
|Number of reagents||Up to 2|
|Dispense volume||5-300 µL|
|Dispense accuracy||≤1% @ 100 µL|
CV ≤1% @ 200 µL
CV ≤3% @ 50 µL
CV ≤5% @ 10 µL
|Power supply||110-120 V 50/60 Hz, or 220-240 V, 50/60 Hz; 175 VA|
|Operating temperature||10-40° C|
|Liquid path materials||Glass, Teflon®, Kel-F|
|Dimensions (W x D x H)||170 x220 x 400 mm|
The Zoom HT is mainly used in production processes. However, it is also useful in research tasks, as the following articles demonstrate:
Tom Woudenberg, Stéphane Pelleau, François Anna, Mikael Attia, Françoise Donnadieu, Alain Gravet, Caroline Lohmann, Hélène Seraphin, Raphaël Guiheneuf, Catherine Delamare, Karl Stefic, Julien Marlet, Etienne Brochot, Sandrine Castelain, Olivier Augereau, Jean Sibilia, François Dubos, Damia Meddour, Christèle Gras-Le Guen, Marianne Coste-Burel, Berthe-Marie Imbert-Marcille, Anne Chauvire-Drouard, Cyril Schweitzer, Amélie Gatin, Sandra Lomazzi, Aline Joulié, Hervé HAAS, Aymeric Cantais, Frederique Bertholon, Marie-France Chinazzo-Vigouroux, Mohamed SI Abdallah, Laurence Arowas, Pierre Charneau, Bruno Hoen, Caroline Demeret, Sylvie Van Der Werf, Arnaud Fontanet, and Michael White
François Anna, Sophie Goyard, Ana Ines Lalanne, Fabien Nevo, Marion Gransagne, Philippe Souque, Delphine Louis, Véronique Gillon, Isabelle Turbiez, François-Clément Bidard, Aline Gobillion, Alexia Savignoni, Maude Guillot-Delost, François Dejardin, Evelyne Dufour, Stéphane Petres, Odile Richard-Le Goff, Zaineb Choucha, Olivier Helynck, Yves L. Janin, Nicolas Escriou, Pierre Charneau, Franck Perez, Thierry Rose and Olivier Lantz
European Journal of Immunology