Brochure of the Centro and Centro XS microplate luminometers
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The Centro supports all basic luminescence technologies including:
Thanks to a stringent selection of photomultipliers working in photon counting mode and more than 40 years of experience building, the Centro provides highly sensitive luminescence detection: less than 5 amol ATP/well (25 fM) can be detected reliably. In the Centro XS, optics have been optimized to increase its sensitivity even further: below 1.8 amol ATP/well (9 fM).
Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples? Then you´re potentially affected by a phenomenon called, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Centro does almost eliminate this problem: with a crosstalk of 10-6, you never have to worry about the position of your high-intensity samples in your plate.
Thanks to its built-in waste pump, you don’t need to worry about loading microplates, waste containers or removal of injector tips for washing and filling the reagent lines; the built-in waste pump takes care of the safe removal of excess liquids.
The Centro can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position*. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence.
Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.
* All injectors in pre-measurement position in the Centro XS.
Choose between different software options according to your application needs:
ICE software is an intuitive, wizard-guided software that walks you through the entire process of setting up your system, collecting your data and reporting the results. The software package is as flexible as your research thanks to the many setting options and freely configurable combinations of operating sequences.
The MikroWin software is a complete software solution providing instrument control, data collection, analysis and reporting. The software is available in different versions, reflecting different user needs. The complete Advance version of the software provides 21 CFR Part 11 compliance and additional QC features like audit trail or configurable user hierarchies.
Low-noise photomultiplier tube in single photon counting mode
|Measurement modes||Endpoint, Kinetics, Repeated, Area scan (up to 100 x 100 points per well)|
|Spectral range luminescence||340-630 nm|
|Injectors||Up to 3 JET injectors with frictionless operation|
|Injector volume||10-100 µL in 1 µL steps|
|Injector accuracy||Better than 2% across the entire volume range|
|Injector precision||Better than 2% across the entire volume range|
|Shaking||Linear, orbital, double orbital, with selectable speed and diameter|
|Temperature control||From ambient +5° to 45° C (not available in Centro XS)|
Centro: <5 amol (25 fM) ATP/well (96-well plate)
Centro XS: <1.8 amol (9 fM) ATP/well (96-well plate)
|Dynamic range||>6 orders of magnitude|
|Interface||Windows software, connected to computer via USB|
|Operating system||Windows 10|
|Available software packages|| |
|Power Supply||100 – 240 VAC ±10%, 50/60 Hz|
|Operating Voltage||24 VDC / 6.67 A|
|Temperature tolerance||Storage: 0-40° C|
Operation: 15-35° C
Maximum relative humidity of 80 % for temperatures up to 31 °C;
decreasing linearly to 50 %, relative humidity up to 40 °C
Dimensions (W x D x H)
|334 x 437 x 287 mm|
There are plenty of articles in the scientific literature using the Centro. Here you have a selection of recent papers.
|2021||Seiya Ozono, Yanzhao Zhang, Hirotaka Ode, Kaori Sano, Toong Seng Tan, Kazuo Imai,Kazuyasu Miyoshi, Satoshi Kishigami, Takamasa Ueno, Yasumasa Iwatani, Tadaki Suzuki & Kenzo Tokunaga||Nature Communications||Pseudotyped virus quantification using Nano-Glo® HiBiT Lytic Detection System|
|2020||Maureen Spit, Nicola Fenderico, Ingrid Jordens, Tomasz Radaszkiewicz,Rik GH Lindeboom, Jeroen M Bugter, Alba Cristobal, Lars Ootes, Max van Osch,Eline Janssen, Kim E Boonekamp, Katerina Hanakova, David Potesil, Zbynek Zdrahal,Sylvia F Boj, Jan Paul Medema, Vitezslav Bryja, Bon-Kyoung Koo, Michiel Vermeulen & Madelon M Maurice||The EMBO Journal||Dual Luciferase|
|2020||Alexandre Quilbe, Olivier Moralès, Martha Baydoun, Abhishek Kumar, Rami Mustapha, Takashi Murakami, Bertrand Leroux, Clémentine de Schutter, Elise Thecua, Laurine Ziane, Ludovic Colombeau, Céline Frochot, Serge Mordon, and Nadira Delhem||Journal of Clinical Medicine||CellTiter-Glo®|
|2020||Sungmi Song, Jin-Young Lee, Ludmila Ermolenko, Aloran Mazumder, Seungwon Ji, Heeju Ryu, HyeJin Kim, Dong-Wook Kim, Jung Weon Lee, Mario Dicato, Christo Christov, Michael Schnekenburger, Claudia Cerella, Déborah Gérard, Barbora Orlikova-Boyer, Ali Al-Mourabit and Marc Diederich||International Journal of Molecular Sciences||GSH/GSSG-Glo™|
|2020||Urooj Fatima, Zhenyu Zhang, Haili Zhang, Xue-Feng Wang, Ling Xu, Xiaoyu Chu,Shuang Ji and Xiaojun Wang||Viruses||Dual Luciferase|
|2020||Stefan Reich, Chi D. L. Nguyen, Canan Has, Sascha Steltgens, Himanshu Soni, Cristina Coman,Moritz Freyberg, Anna Bichler, Nicole Seifert, Dominik Conrad, Christiane B. Knobbe-Thomsen,Björn Tews, Grischa Toedt, Robert Ahrends & Jan Medenbach||Nature Communications||NADP/NADPH-Glo™|
|2020||Yeojung Nam, Kyung Min Kang, Se Ra Sung, Ji Eun Park, Yun-Jeong Shin, Seung Hun Song, Ju Tae Seo, Tae Ki Yoon, Sung Han Shim||Asian Journal of Andrology||Dual Luciferase|
|2020||Monica Z. Wu, Haruichi Asahara, George Tzertzinis, and Bijoyita Roy||RNA||BioLux® Cypridina Luciferase Assay|
|2020||Da Hee Lee, Na Eun Kwon, Won-Ji Lee, Moo-Seung Lee, Doo-Jin Kim, Ji Hyung Kim and Sung-Kyun Park||Cells||Glucose Uptake-Glo™|
|2020||Qingkang Lyu, Irene S. Ludwig, Peter J. S. Kooten, Alice J. A. M. Sijts, Victor P. M. G. Rutten, Willem van Eden, Femke Broere||Cell Stress and Chaperones||Steady-Glo®|
|2020||Daichi Hayashi, Toshiharu Shirai, Ryu Terauchi, Shinji Tsuchida, Naoki Mizoshiri, Yuki Mori, Yuji Arai, Osam Mazda, and Toshikazu Kubo||Oncology Letters||RealTime-Glo™ MT Cell Viability Assay|
|2020||Stefano Del Prato, Jahoon Kang, Michael E. Trautmann, John Stewart, Christopher H. Sorli, Michael Derwahl, Alfonso Soto and Kun‐Ho Yoon||Diabetes, Obesity and Metabolism||cAMP-Glo™|
|2020||Vito Lampasona, Massimiliano Secchi, Marina Scavini, Elena Bazzigaluppi, Cristina Brigatti, Ilaria Marzinotto, Alberto Davalli, Amelia Caretto, Andrea Laurenzi, Sabina Martinenghi, Chiara Molinari, Giordano Vitali, Luigi Di Filippo, Alessia Mercalli, Raffaella Melzi, Cristina Tresoldi, Patrizia Rovere-Querini, Giovanni Landoni, Fabio Ciceri, Emanuele Bosi and Lorenzo Piemonti||Diabetologia||LIPS with NanoLuc® Luciferase|
|2019||Jun Weng, Xu Han, Kaiyu Liu, Jiong Yang, Shiruo Wei, Yue Zhang, Fanhong Zeng, Yang Li, Li Shen, Yi Gao||International Journal of Biological Sciences||Dual-Glo®|
You can find additional publications which have used Centro instruments in publication databases.
Please keep in mind that the list provided is not exhaustive, but it offers valuable examples of experiments performed with our instruments and published in peer-reviewed journals.
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Berthold Technologies GmbH & Co.KG
Calmbacher Str. 22
75323 Bad Wildbad
Tel.: (+49) 7081 177 0
Fax: (+49) 7081 177 100