Application Notes

BRET Assay for 7TM Receptors Using the Mithras A Functional BRET Assay for 7TM Receptors Using the Mithras LB 940 Multimode Plate Reader

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Measurement of cAMP using AlphaScreen® with the Mithras Mithras LB 940 Multimode Plate Reader Used for AlphaScreen® Signal Detection: Measurement of cAMP Production Induced by a G-protein Coupled Receptor

PDF | 322.9 KB

Interaction between ER alpha and SCR-1 NR-2 through FP with Mithras Characterization of the interaction between recombinant estrogen receptor alpha and transcriptional coactivator SCR-1 NR-2 binding motif through Fluorescence Polarization study with Mithras LB 940

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BRET-based studies of receptor dynamics with Mithras Bioluminescence Resonance Energy Transfer (BRET)-based studies of receptor dynamics in living cells with Berthold’s Mithras Multimode Microplate Reader

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BRET to monitor dynamic receptor-protein interactionswith the Mithras Bioluminescence Resonance Energy Transfer (BRET) as a means of monitoring dynamic receptor-protein interactions in living cells measured on LB 940 Mithras Multimode Microplate Reader

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Basic Considerations for BRET Assays with the Mithras Basic Considerations for Bioluminescence Resonance Energy Transfer (BRET) Assays for G-protein coupled receptor protein interactions in living cells

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Calcium monitoring with the Mithras Calcium monitoring using Fura-2 with the Mithras LB 940 multimode plate reader

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ADP Quest™ HS with Mithras ADP Quest™ HS kinetic assay with the Mithras LB 940 Multimode Microplate Reader

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PhosphoQuest™ assay with Mithras PhosphoQuest™ cell-based ELISA kinase assay with Mithras LB 940 Multimode Reader

PDF | 369.6 KB

HitHunter™ cAMP HS+ assay with Mithras HitHunter™ cAMP HS+ assay with Mithras LB 940 Multimode Microplate Reader

PDF | 515.2 KB

PathHunter™ ß-arrestin assay with Mithras PathHunter™ ß-arrestin luminescence assay with Mithras LB 940 Multimode Microplate Reader

PDF | 255.0 KB

PathHunter™ ß-arrestin flash assay with Mithras PathHunter™ ß-arrestin flash luminescence assay with Mithras LB 940 Multimode Microplate Reader

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PathHunter™ secretion assay with Mithras PathHunter™ secretion luminescent assay with Mithras LB 940 Multimode Microplate Reader

PDF | 274.2 KB

Cell viability assays with Mithras Cell viability assays with Mithras LB 940 Multimode Microplate Reader

PDF | 1.5 MB

cAMP/IP-One HTplex HTRF® assay with Mithras cAMP/IP-One HTplex cell-based experiment performed on Mithras LB 940 Multimode Microplate Reader using HTRF® technology

PDF | 686.6 KB

Comparison of filter sets for BRET1 using Mithras Comparison of filter sets for BRET1 assays: ß-arrestin2 (ßARR2) recruitment to the vasopressin V2 receptor using the Mithras LB 940 Multimode Microplate Reader

PDF | 187.6 KB

TRANSIL® Brain Absorption Assay with Mithras TRANSIL® Brain Absorption Assay with Mithras LB 940 Multimode Microplate Reader

PDF | 251.3 KB

Cytochrome P450-3A4 Inhibition Assay using Hudson Robotics’ VaryScreen ADME and Mithras Measuring Cytochrome P450-3A4 Inhibition Assay on Hudson Robotics’ VaryScreen ADME Equipped with the Mithras Multimode Microplate Reader

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Measurement of ROS in Arabidopsis and N. benthamiana with Mithras Measurement of Reactive Oxygen Species (ROS) in Arabidopsis and N. benthamiana plants with the Mithras Multimode Microplate Reader

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FRET to Study GPCR Oligomerization with Mithras FRET as a Tool to Study G-protein Coupled Receptor Oligomerization in HEK Cells.

PDF | 397.9 KB

Study of properties of nucleic acid-dependent photosensitizer using Mithras Study of properties of nucleic acid dependent photosensitizer In(III)(pyropheophorbide-a)chloride by using a LB 940 Mithras plate reader coupled to a red diode array light source

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Listeria and Salmonella Detection using Crocodile and Orion II 24 h Listeria and Salmonella Detection in food matrices using rRNA based LUMIprobe 24 Method with the Crocodile Assay miniWorkstation and the Orion II Microplate Luminometer

PDF | 251.9 KB

Intracellular Ca2+ with Clonetics™ Primary Sensors and the Orion II Monitoring Intracellular Ca2+ Fluxes with Clonetics™ Primary Sensors using the Orion II Microplate Luminometer

PDF | 333.6 KB

ABEL® Cell Activation Assay with the Orion II ABEL® Cell Activation Assay for Whole Blood or Isolated Cells with Pholasin® and Adjuvant-K™ with the Orion II Microplate Luminometer

PDF | 255.9 KB

ViaLight™ Assay with the Orion II ViaLight™ Cell proliferation and Cytotoxicity BioAssay Kit (Lonza) with the Orion II Microplate Luminometer

PDF | 67.5 KB

ToxiLight™ Cytotoxicity Assay with the Orion II ToxiLight™ Non-Destructive Cytotoxicity Bio Assay Kit (Lonza) with the Orion II Microplate Luminometer

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MycoAlert® Assay with the Orion II MycoAlert® Mycoplasma Detection Assay (Lonza) with the Orion II Microplate Luminometer

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Abel® Antioxidant Assay with the Orion II Abel® Antioxidant Assay with Superoxide with the Orion II Microplate Luminometer

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ATP measurements with the Orion II Comparison of standard curves for ATP with the Orion II Microplate Luminometer

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DLR Assay (Promega) with the Orion II Dual Luciferase Reporter Assay (Promega) with the Orion II Microplate Luminometer

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Luciferase Reporter Assay with the Orion II Luciferase Reporter Assay with the Orion II Microplate Luminometer

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ABEL® Cell Activation Assay with the Sirius L ABEL® Cell Activation Assay for Whole Blood or Isolated Cells with Pholasin® and Adjuvant-K™ using the Sirius L Tube Luminometer

PDF | 175.4 KB

Rapid Bacterial Detection with the Sirius L Rapid Bacterial Detection Assay (Useful Biology) with the Sirius L Tube Luminometer

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ATP detection limit with Sirius L Below amol ATP detection limit with Sirius L Tube Luminometer and ATP Reagent SS from BioThema

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SingleQuant protein quantification assay with the Colibri Microvolume protein quantification based on Amido Black (SingleQuant Assay) with the Colibri Microvolume Spectrophotometer

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RNA quantification with the Colibri Low volume measurement of RNA using the Colibri Microvolume Spectrophotometer

PDF | 170.5 KB

Measurement of ssDNA using the Colibri Low volume measurement of ssDNA using the Colibri Microvolume Spectrophotometer for DNA quantification

PDF | 92.3 KB

Analysis of circadian rhythms using NightShade Improved experimental setup for analysis of circadian rhythms using the NightShade Plant In Vivo Imaging System

PDF | 479.1 KB

Circadian Clock with NightSHADE Getting to Know The Circadian Clock and Plant Growth With NightSHADE

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Measurement of Biophoton Emission with NightShade Measurement of Biophoton Emission in Plants – An Alternative Monitoring System for Stress Factors

PDF | 1.4 MB

Measurement of delayed fluorescence with NightShade Measurement of Delayed Fluorescence in Plants - a Monitoring System for Stress Factors

PDF | 560.7 KB

Fluorescence in different subcellular localizations with NightShade Detection of Fluorescence in Transgenic Arabidopsis Lines Expressing Fluorophores in Different Subcellular Localizations

PDF | 840.6 KB

Trans-illumination in GFP-Expressing Plants using NightShade Trans-illumination – a Solution for Excitation of GFP Expressing Plants in Petri Dishes for In Vivo Imaging using the NightShade Plant In Vivo Imaging System

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Fluorescent nanoparticle imaging using NightOWL Fluorescence in Vivo Imaging of labeled nanoparticles in tumour-bearing mice with NightOWL II In Vivo Imaging System

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Bioluminescence Imaging using NightOWL Bioluminescence Imaging using NightOWL LB 981 NC 100. Cells expressing luciferase gene under the control of a constitutive promoter were used as a model of in vivo proliferation of cancer cells.

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Luciferin bioavailability in mice during in-vivo imaging To determine the optimal luciferase activity detection time, time course experiments were performed.

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Monitoring of Renilla Luciferase Activities in-vitro and in-vivo The expression of a novel EnduRen™ and ViviRen™ Renilla luciferase reporter gene (Promega) in vitro and in vivo is explored

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Quantum dots in Molecular Imaging using NightOWL Qdots® molecular imaging is a new way of seeing biologic processes at work within cells and in small animals.

PDF | 529.5 KB

Bioo Maxsignal® Aflatoxin M1 ELISA Test Kit with the Crocodile Automation of the Bioo Maxsignal® Aflatoxin M1 ELISA Test Kit using the Crocodile miniWorkstation

PDF | 860.5 KB

IDEXX Milk Pregnancy Test with the Crocodile Automation of the IDEXX Milk Pregnancy Test using the Crocodile ELISA miniWorkstation

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Demeditec Cortisol free in Saliva ELISA kit with the Crocodile Automation of the Demeditec Cortisol free in Saliva ELISA kit using the Crocodile ELISA miniWorkstation

PDF | 548.5 KB

Sedium R&D BSA ELISA kit with the Crocodile Diagnostic Sensitivity and Specificity of samples tested with the Crocodile ELISA miniWorkstation and Bovine Serum Albumin ELISA Kit from Sedium R&D

PDF | 333.3 KB

Enzo APP ΔC31 ELISA with the Crocodile Automation of the Enzo APP ΔC31 ELISA kit using the Crocodile ELISA miniWorkstation

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Analytical sensitivity using PrioCHECK® Toxoplasma Ab porcine ELISA with the Crocodile Analytical sensitivity of samples tested with the Crocodile ELISA miniWorkstation in comparison to hand processing using PrioCHECK® Toxoplasma Ab porcine ELISA from Prionics AG

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Listeria and Salmonella Detection in food matrices using Crocodile and Orion II 24 h Listeria and Salmonella Detection in food matrices using rRNA based LUMIprobe 24 Method using the Crocodile Assay miniWorkstation and the Orion II Microplate Luminometer

PDF | 251.9 KB

Sension Progesterone Milk ELISA using the Crocodile miniWorkstation Precision, Repeatability, Recovery and Linearity of the Sension Progesterone Milk ELISA using the Crocodile ELISA miniWorkstation

PDF | 697.0 KB

Invitron Intact Proinsulin ELISA using the Crocodile Automation of the Invitron Intact Proinsulin ELISA using the Crocodile miniWorkstation

PDF | 134.9 KB

Reverse Hybridisation of DNA probe array with Crocodile Reverse Hybridisation of DNA probe array with Crocodile Assay miniWorkstation and measurement using an ELISpot reader

PDF | 235.9 KB

Serotonin ELISA from DLD Diagnostika using the Crocodile Automation of the DLD Diagnostika Serotonin ELISA using the Crocodile miniWorkstation

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Diagnostic Sensitivity and Specificity of PrioCHECK® Toxoplasma Ab porcine ELISA using Crocodile Diagnostic Sensitivity and Specificity of samples tested with the Crocodile miniWorkstation and PrioCHECK® Toxoplasma Ab porcine ELISA from Prionics AG

PDF | 93.8 KB

Repeatability and Reproducibility of PrioCHECK® Toxoplasma Ab porcine ELISA using the Crocodile Repeatability and Reproducibility of tests run on the Crocodile miniWorkstation using PrioCHECK® Toxoplasma Ab porcine ELISA from Prionics AG

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