Top tips and tricks: performing assays in microplates
Microplate assays are now a standard analytical tool in many life science experiments, matched by the corresponding growth of the types of microplate readers available to conduct critical measurements.
From absorbance and fluorescence to protein-protein interactions and the quantification of difficult-to-measure proteins, technology providers are developing microplate readers to overcome common problems and offer gains in measurement sensitivity, reliability, speed, and automation.
Choosing the right assay is vital, but equally important is an understanding of how microplates themselves can affect measurement through phosphorescence, or how detection technologies which operate in the center of wells can be unreliable for any non-homogeneous sample, unless corrective measures are taken. Mastering common setbacks with the right technology is the key to fast, reliable, and accurate microplate assays.
Choose the best microplate reader for your work, optimize current processes, and avoid setbacks with this expert guide.