It is recommended to use the Prime function to fill the injector lines with deionized water first. Then attach the reagents to the injector lines and fill the lines with the reagent using the Prime function again. This procedure prevents splashing and contamination of the injector tips with reagents which may occur if an air/reagent mixture would leave the injector tips. This also ensures that there is >99% homogenous mix of reagent at the tip for proper injections across the whole plate.
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