To evaluate the polarization two emission measurements are needed: the first using a polarized emission filter parallel to the excitation filter (S-plane) and the second with a polarized emission filter perpendicular to the excitation filter (P-plane). Typical labels are FITC, BODIPY or TMR (TRITC).
Application Notes related to FP
Interaction between ER alpha and SCR-1 NR-2 through FP with Mithras Characterization of the interaction between recombinant estrogen receptor alpha and transcriptional coactivator SCR-1 NR-2 binding motif through Fluorescence Polarization study with Mithras LB 940
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