Brochure of the Zoom HT LB 920 Microplate Washer
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It takes only 17 seconds for a triple wash of a 96 well microplate. Combined with the unique one-rail plate transfer design which moves the plate a very short distance from the stack to the wash position. This enables a typical throughput of 150 washed plates per hour, or up to 1050 plates per day (3 x 300 µL, 7 h per day, 1 single Zoom HT).
Each wash channel has its own aspirate and dispense tip, which allows simultaneous washing of all 96 wells. The same 96 channel wash head is used for 384 well washing in four quadrants, making it unnecessary to purchase different wash heads for different plate types. Rapid positioning of the wash head provides 384 well wash speeds comparable to 384 well channel washers. Large diameter aspirate tips, 3-dimensional (3D) tip positioning and a constant flow rate vacuum pump ensure a smooth and efficient washing experience.
Two wash modes are available: Regular wash mode and Superwash mode (also known as “Overflow mode”). When using the regular wash mode, the wash fluid that is dispensing and evacuated follow each other. When using the Superwash mode, the wash fluid that is dispensing and evacuated are conducted simultaneously creating a continuous wash fluid flow throughout the entire well. The Zoom HT supports creating and storing of the 3D tip positioning profile for each plate type and assay. This profile defines the aspirate tip horizontal and vertical positions thereby mapping how the well is aspirated. In addition, the speed with which the tip is immersed into the well is adjustable. Fine-tuning of all the parameters of the wash procedure will allow to wash even loosely adherent cultured cells. The Zoom HT will have residual wash fluid volumes of less than 1 μL/well, this can be achieved when a surfactant is present in the wash fluid.
The discarded wash fluids are captured by a self-emptying waste trap, which allows uninterrupted processing of large plate batches. Biohazard waste management is supported by the automatic addition of a decontamination solution which is continuously drained from the trap.
The Zoom HT is available in 3 different configurations using a one, two or four wash buffer inlet. This is advantageous for applications that require sequential cleaning with different buffers or for an automatic cleaning routine after using the system.
The unique design of the dispense manifold eliminates the need for any special cleaning. The aspirate head is maintenance free because of the large aspirate tip diameter. This is combined with a continuous vacuum that reliably prevents any accumulation of reagent residue.
The optional Dispense Module is an easy-to-install plug-and-play device, is extremely easy to use and requires no calibration. With the addition of a dispense module the Zoom HT Washer becomes an efficient washer-dispenser combination system. The Dispensing Module provides single or dual-channel dispensing of reagents using direct drive and positive displacement syringes to ensure the highest precision and accuracy when dispensing. The dispense module feeds one or two 8- or 16-way manifolds for 96 and/or 384 well microplates.
96 channel wash head for fast processing of 96 and 384 well microplates
|Wash modes||Standard and Superwash|
|Wash fluid selection||1, 2 or 4 inlet versions|
|Wash volume||5-300 µL|
|Aspirate tip descent speeds||Slow, Medium, Fast|
|Soak time||0-99 s|
|Shaking||Up to 80 s, amplitude 1 mm, frequency 12 Hz|
|Number of programs||Up to 99|
|Wash cycles per program||Up to 99|
|Program protection||Administrator mode to prevent unauthorized changes|
96-well plates and strips, 384-well plates; dimensions according to ANSI SLAS
One-rail design connecting wash, dispense and storage positions; plate capacity: 30 plates per tower
|Wash head dispense accuracy|
±2% typical, 50-300 µL
|Wash head dispense precision|
CV ≤2.5% @ 200 µL
CV ≤3% @ 100 µLCV ≤4.5% @ 50 µL
|Residual volume||<2 µL/well|
|Plate processing speed|
96-well plate, 300 µL (1 cycle), including stack: 14 s
96-well plate, 300 µL (3 cycles), including stack: 24 s384-well plate, 75 µL (3 cycles), including stack: 44 s
|Power supply||110-120 V 50/60 Hz, or 220-240 V, 50/60 Hz; 300 VA|
|Operating temperature||10-40° C|
|Liquid path materials|
Stainless steel, Teflon®, PVC, Norprene, Silicone, Polysulfone
|Dimensions (W x D x H)||690 x 560 x 610 mm|
8-way and 16-way manifolds available
|Dispense manifold ports||2 in direct proximity to wash head for immediate dispensing after aspiration|
|Syringe size||2 x 10 mL, separate channels|
|Dispensing technology||Positive displacement|
|Number of reagents||Up to 2|
|Dispense volume||5-300 µL|
|Dispense accuracy||≤1% @ 100 µL|
CV ≤1% @ 200 µL
CV ≤3% @ 50 µL
CV ≤5% @ 10 µL
|Power supply||110-120 V 50/60 Hz, or 220-240 V, 50/60 Hz; 175 VA|
|Operating temperature||10-40° C|
|Liquid path materials||Glass, Teflon®, Kel-F|
|Dimensions (W x D x H)||170 x220 x 400 mm|
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Berthold Technologies GmbH & Co.KG
Calmbacher Str. 22
75323 Bad Wildbad
Tel.: (+49) 7081 177 0
Fax: (+49) 7081 177 100