Brochure of the Tristar 3 and Tristar 5 Multimode Microplate Readers
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The Tristar 3 Multimodeprovides you with application flexibility for today, tomorrow, and beyond to master your changing plate reading applications:
From UV absorbance for DNA quantification to measurements in the far red or NIR (in selected configurations), the Tristar 3 is ready for any assay and greatly improves the wavelength range of the older TriStar².
All Tristar 3 preconfigured models have the features you need to perform BRET assays: best-in-class luminescence sensitivity and a high quality filter system. To start working with BRET, just order the upgrade for your specific assay: BRET1, BRET2, NanoBRET™ or others.
Our patented ONE-4-ALL optics have been optimised to combine the stability and user-friendliness of a multimodal optical system with the sensitivity and versatility of dedicated optical devices.
ONE-4-ALL optics work for both filter- and monochromator-based applications, so there´s never a compromise in performance in any mode.
The quick-change filter technology of the Tristar series provides you with the flexibility required to meet your application needs: up to 40 different excitation and emission filters can be easily mounted on exchangeable filter slides.
The filters are characterized by high transmissions properties which can be up to 25-fold that of monochromators. Technologies likecan be measured more efficiently with filters.
Furthermore, filters with a large bandwidth are available to analyze fluorophores with wide spectra and all luminescence-based assay requiring to use filters, e.g.assays.
The ergonomic design of the system provides full front access for all key operations (e.g. plate loading, filter change and connecting reagents). Reagent vials can be stored in an integrated front compartment, providing easy access and visibility. It contains a removable trough that can be filled with water or ice to keep all reagents cooled. Different reagent tube holders are available to ensure secure handling of valuable reagents. The system has a flat surface on its top, providing enough space to put down a laptop.
Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples? Then you´re potentially affected by a phenomenon called, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Tristar 3 does almost eliminate this problem: with a crosstalk of 10-6, you never have to worry about the position of your high-intensity samples in your plate.
The Tristar 3 can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence.
Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.
Choose between different software options according to your application needs:
ICE software is an intuitive, wizard-guided software that walks you through the entire process of setting up your system, collecting your data and reporting the results. The software package is as flexible as your research thanks to the many setting options and freely configurable combinations of operating sequences.
The MikroWin software is a complete software solution providing instrument control, data collection, analysis and reporting. The software is available in different versions, reflecting different user needs. The complete Advance version of the software provides 21 CFR Part 11 compliance and additional QC features like audit trail or configurable user hierarchies.
For many laboratories, validation, qualification and ensuring compliance with a number of GMP and GLP requirements is essential. We provide a number of tools and services to help you ensure that your system is running at peak performance.
We provide a variety of tools to help you check and confirm the performance of your products over time.
Berthold Expert Services provide a team of dedicated and factory-trained engineers and experts to optimize your productivity. We and our local partners are always at your disposal.
Our team of technical support scientists is your partner to overcome the unique challenges your application brings. Contact our team to discuss your assay or experiment design, data analysis questions, troubleshooting and more.
|Detection technologies|| |
|Measurement modes||Endpoint, Kinetics, Repeated, Spectral scan, Area scan (up to 100 x 100 points per well)|
|Filters||High quality interference filters|
|Filter handling||Easy to change filter slides with up to 5 filters per slide, additional slides available|
|Optical system||ONE-4-ALL optics|
Xenon flash lamp
|Spectral range Absorbance||200-1000 nm|
|Spectral range Luminescence|
Standard: 280-650 nm
FL models: 280-850 nm
|Spectral range Fluorescence|
Excitation: 200-1000 nm
Emission, standard: 280-650 nm
Emission, FL models: 280-850 nm
|Injectors||Up to 3 JET injectors with frictionless operation|
|Injector volume||10-100 µL in 1 µL steps|
|Injection speed||200 – 440 μL/sec|
|Injector accuracy||Better than 2% across the entire volume range|
|Injector precision||Better than 2% across the entire volume range|
|Microplate formats||6- to 384-well|
|Plate height adjustment||3 different frames depending on plate type|
|Additional sample formats (optional)|| |
|Shaking||Linear, orbital, double orbital, with selectable speed and diameter|
|Temperature control||From ambient +5° to 45° C, includes additional cooling for the photomultiplier|
|Gas connection||Yes (optional)|
|Absorbance precision||Better than 0.6%|
|Absorbance accuracy||Better than 2%|
|Luminescence sensitivity||<6 amol (0.03 pM) ATP/well (96-well plate)|
|Fluorescence sensitivity||<7 amol Fluorescein/well (384-well small volume plate)|
|Crosstalk||10-6 (black plates)|
|Dynamic range||>6 orders of magnitude|
|Interface||Windows software, connected to computer via USB|
|Operating system||Windows 10|
|Available software packages|| |
110-240 VAC ± 10%
|Operating Voltage||24 VDC ± 5%|
|Power consumption||140 VA|
|Temperature tolerance||Storage: 0-40° C|
Operation: 15-35° C
|max 2000 m (above sea level)|
Dimensions (W x D x H)
391 x 470 x 344 mm
approx. 20 kg
The following Application Notes were created with earlier instrument versions of the Tristar system family, but can be transferred 1:1 to the new Tristar systems.
Screening of GPCR antagonists using Ca2+ measurements and the TriStar² S 3 commercially available compounds are screened for P2Y2 receptor inhibition using the calcium mobilisation assay with two different fluorescent dyes measured on a TriStar² LB 942 multimode microplate reader.
PDF | 1,009.5 KB
Monitoring circadian gene expression in micro-algae using the TriStar² We report how the Berthold TriStar² multimode microplate reader equipped with luminescence module, in combination with an external LED illumination adaptor, can be used to image circadian rhythms in the micro algae Ostreococcus tauri.
PDF | 675.1 KB
The Tristar 3 is a very recent instrument and thus not many papers using it have yet been published. Here you have a selection of recent articles.
|2022||Ryotaro Nouda, Shohei Minami, Yuta Kanai, Takahiro Kawagishi, Jeffery A. Nurdin, Moeko Yamasaki, Ryusei Kuwata, Hiroshi Shimoda, Ken Maeda, and Takeshi Kobayashi||PNAS||Nano-Glo® HiBiT Lytic Detection System|
Long Cheng, Yan Wang, Jiawei Wang, Haibo Qin, Guangbiao Zhu, and Lingsong Tao
J Healthc Eng
MTS, ATP bioluminescent somatic cell assay kit
You can find additional publications which have used older Tristar instruments in publication databases.
Please keep in mind that the list provided is not exhaustive, but it offers valuable examples of experiments performed with our instruments and published in peer-reviewed journals.
Tristar 3 Research
Tristar 3 Research Plus
Tristar 3 Research FL
Tristar 3 Research Plus FL
|Fluorescence (incl. FRET) up to 650 nm|
|Fluorescence (incl. FRET) up to 850 nm|
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Berthold Technologies GmbH & Co.KG
Calmbacher Str. 22
75323 Bad Wildbad
Tel.: (+49) 7081 177 0
Fax: (+49) 7081 177 100