Brochure of the Tristar 3 and Tristar 5 Multimode Microplate Readers
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The Tristar 5 Multimode Microplate Reader provides you with application flexibility for today, tomorrow, and beyond to master your changing plate reading applications. The system supports the following wide range of detection modes:
The Tristar 5 system is equiped with FlexTec Optics, offering you the best of two worlds – benefit from the flexibility to easily select a discrete wavelength and perform spectral scans for both, excitation and emission using its built-intechnology. Or optimize your assays´ sensitivity by utilizing the system filter sliders for excitation and emission. Or mix both technologies if required: the Tristar 5 delivers both, flexibility and sensitivity in a single system.
The Tristar 5 employs up to two double monochromators providing blocking properties needed in sensitive fluorescence assays. Both monochromators are equipped with software driven continuous bandwidth variation to optimise the instrument for the specific demands of different assay conditions.
Berthold´s flexible monochromator technology offers you variable bandwidths from 4-12 nm in excitation and 8 – 22 nm for emission, selectable in 1 nm increments.
From UV absorbance for DNA quantification to measurements in the far red or NIR (in selected configurations), the Tristar 5 is ready for any assay.
The Tristar 5 enables you to read both, from below as well as from the top with high-performance. The advancedof the system can be easily selected via the software to help you analyse e.g. adherent cells and obtain best sensitivity and a superior signal-to-background ratio for these applications.
Our patented ONE-4-ALL optics have been optimised to combine the stability and user-friendliness of a multimodal optical system with the sensitivity and versatility of dedicated optical devices.
ONE-4-ALL optics work for both filter- and monochromator-based applications, so there´s never a compromise in performance in any mode.
The quick-change filter technology of the Tristar series provides you with the flexibility required to meet your application needs: up to 40 different excitation and emission filters can be easily mounted on exchangeable filter slides.
The filters are characterized by high transmissions properties which can be up to 25-fold that of monochromators. Technologies likecan be measured more efficiently with filters.
Furthermore, filters with a large bandwidth are available to analyze fluorophores with wide spectra and all luminescence-based assay requiring to use filters, e.g.or NanoBRET™ assays.
The ergonomic design of the system provides full front access for all key operations (e.g. plate loading, filter change and connecting reagents). Reagent vials can be stored in an integrated front compartment, providing easy access and visibility. It contains a removable trough that can be filled with water or ice to keep all reagents cooled. Different reagent tube holders are available to ensure secure handling of valuable reagents. The system has a flat surface on its top, providing enough space to put down a laptop.
Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples? Then you´re potentially affected by a phenomenon called, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Tristar 5 does almost eliminate this problem: with a crosstalk of 10-6, you never have to worry about the position of your high-intensity samples in your plate.
The Tristar 5 can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence.
Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.
Choose between different software options according to your application needs:
ICE software is an intuitive, wizard-guided software that walks you through the entire process of setting up your system, collecting your data and reporting the results. The software package is as flexible as your research thanks to the many setting options and freely configurable combinations of operating sequences.
The MikroWin software is a complete software solution providing instrument control, data collection, analysis and reporting. The software is available in different versions, reflecting different user needs. The complete Advance version of the software provides 21 CFR Part 11 compliance and additional QC features like audit trail or configurable user hierarchies.
For many laboratories, validation, qualification and ensuring compliance with a number of GMP and GLP requirements
is essential. We provide a number of tools and services to help you ensure that your system is running at
We provide a variety of tools to help you check and confirm the performance of your products over time.
Berthold Expert Services provide a team of dedicated and factory-trained engineers and experts to optimize your productivity. We and our local partners are always at your disposal.
Our team of technical support scientists is your partner to overcome the unique challenges your application brings. Contact our team to discuss your assay or experiment design, data analysis questions, troubleshooting and more.
|Detection technologies (configuration-dependent)|| |
|Measurement modes||Endpoint, Kinetics, Repeated, Spectral scan, Area scan (up to 100 x 100 points per well)|
|Bottom reading||Yes (configuration-dependent)|
|Filters||High quality interference filters|
|Filter handling||Easy to change filter slides with up to 5 filters per slide, additional slides available|
|Monochromator||Optional double 3D monochromator for absorbance, excitation and emission, high transmission (F number: 2.7)|
|Monochromator bandwidth||Software-controlled variable bandwidth, 4-22 nm (1 nm increments)|
|Monochromator stray light||10-6|
|Optical system||ONE-4-ALL optics|
Xenon flash lamp
|Spectral range Absorbance||200-1000 nm|
|Spectral range Luminescence|
Standard: 280-650 nmFL models: 280-850 nm
|Spectral range Fluorescence|
Excitation: 230-1000 nm
Emission, standard: 280-650 nmEmission, FL models: 280-850 nm
|Injectors||Up to 3 JET injectors with frictionless operation|
|Injector volume||10-100 µL in 1 µL steps|
|Injection speed||200 – 440 μL/sec|
|Injector accuracy||Better than 2% across the entire volume range|
|Injector precision||Better than 2% across the entire volume range|
|Microplate formats||6- to 384-well|
|Plate height adjustment||3 different frames depending on plate type|
|Additional sample formats (optional)|| |
|Shaking||Linear, orbital, double orbital, with selectable speed and diameter|
|Temperature control||From ambient +5° to 45° C, includes additional cooling for the photomultiplier|
|Gas connection||Yes (optional)|
|Absorbance precision||Better than 0.6%|
|Absorbance accuracy||Better than 2%|
|Luminescence sensitivity||<6 amol (0.03 pM) ATP/well (96-well plate)|
|Fluorescence sensitivity||<7 amol Fluorescein/well (384-well small volume plate)|
|TRF sensitivity||<5 amol Eu|
|Crosstalk||10-6 (black plates)|
|Dynamic range||>6 orders of magnitude|
|Interface||Windows software, connected to computer via USB|
|Operating system||Windows 10|
|Available software packages|| |
110-240 VAC ± 10%
|Operating Voltage||24 VDC ± 5%|
|Power consumption||140 VA|
|Temperature tolerance||Storage: 0-40° C|
Operation: 15-35° C
|Altitude||max 2000 m (above sea level)|
Dimensions (W x D x H)
391 x 470 x 395 mm
approx. 32 kg
The following Application Notes were created with earlier instrument versions of the Tristar system family, but can be transferred 1:1 to the new Tristar systems.
Transcreener® Fluorescence Intensity Assay with the TriStar² S The compatibility of the Transcreener® Fluorescence Intensity Assay Kit for ADP is tested with the TriStar² S LB 942 Multimode Microplate Reader.
PDF | 1.0 MB
Transcreener® GDP TR-FRET Red Assay with TriStar² S Validation of the TriStar² S Multimode Microplate Reader with the Transcreener® GDP TR-FRET Red assay
PDF | 934.0 KB
NanoBRET™ with the TriStar² S Promega NanoBRET™ protein:protein interaction system with the TriStar² S Multimode Microplate Reader
PDF | 396.4 KB
Screening of GPCR antagonists using Ca2+ measurements and the TriStar² S 3 commercially available compounds are screened for P2Y2 receptor inhibition using the calcium mobilisation assay with two different fluorescent dyes measured on a TriStar² LB 942 multimode microplate reader.
PDF | 1,009.5 KB
Monitoring circadian gene expression in micro-algae using the TriStar² We report how the Berthold TriStar² multimode microplate reader equipped with luminescence module, in combination with an external LED illumination adaptor, can be used to image circadian rhythms in the micro algae Ostreococcus tauri.
PDF | 675.1 KB
The Tristar 5 is a very recent instrument and thus not many papers using it have yet been published. Here you have a selection of recent articles.
|2020||Yujun Li, Haimin Wang, Xiaojuan Tang, Shisong Fang, Danting Ma, Chengzhi Du, Yifei Wang, Hong Pan, Weitong Yao, Renli Zhang, Xuan Zou, Jie Zheng, Liangde Xu, Michael Farzan, Guocai Zhonga||Journal of Virology||using Gaussia|
You can find additional publications which have used older Tristar instruments in publication databases.
Please keep in mind that the list provided is not exhaustive, but it offers valuable examples of experiments performed with our instruments and published in peer-reviewed journals.
Tristar 5 Research
Tristar 5 Research Plus
Tristar 5 Research FL
Tristar 5 Research Plus FL
Tristar 5 Research Performance FL
|Fluorescence (incl. FRET) up to 650 nm|
|Fluorescence (incl. FRET) up to 850 nm|
|Fluorescence Polarization (FP)||optional||optional||optional||optional||optional|
|Wavelength selection - Excitation||monochromator or filter||monochromator or filter||monochromator or filter||monochromator or filter||monochromator or filter||monochromator or filter||monochromator or filter||monochromator or filter|
|Wavelength selection - Emission||filter||filter||filter||filter||filter||monochromator or filter||monochromator or filter||monochromator or filter|
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Berthold Technologies GmbH & Co.KG
Calmbacher Str. 22
75323 Bad Wildbad
Tel.: (+49) 7081 177 0
Fax: (+49) 7081 177 100