AutoLumat LB 953 Multi-Tube Luminometer

Automation for tubes

The automatic tube luminometer AutoLumat is an unique instrument for the measurement of bio- and chemiluminescence applications

  • Reporter Gene assays
  • ATP monitoring
  • luminescent immunoassays (LIA)
  • enzyme measurements
  • cellular luminescence / oxidative burst

Up to 164 samples placed in a chain can be measured once or sequentially many times over a long period.

 

Tabs

Features

Peltier-cooled detector

Peltier cooling of the detection unit ensures extremely low and stable background noise resulting in unsurpassed sensitivity.

Variable volume injectors

Based on the proprietary JET injection technology up to 3 injectors combine high speed and reliable mixing of reagents with outstanding precision. The injected volume can be adjusted within the range 25 to 300 µL. The materials used are able to withstand even corrosive substances which are often used in chemiluminescence assays.

Temperature control

The tube compartment of the instrument can be temperature controlled making the AutoLumat the instrument of choice for cellular luminescence assays.

Software

The user can choose to control the instrument via the built-in software or the PC software Tubemaster.

  • single measurement
  • Dual measurement (e.g. dual reporter gene assays)
  • kinetic measurements
  • cut-off protocol
  • immunoassay (LIA, ILMA) evaluation
Specifications

Detection Unit

ultra low noise photomultiplier in single photon counting mode

spectral range 380 to 630 nm

Peltier-cooled

Tube Formats

12 x 75 mm (Ø x h)

12 x 55 mm (Ø x h)

Sample Capacity164 tubes
Temperature Control5 °C above RT to 42 °C (option)
Injection

up to 3 JET injectors

variable volume 25 to 300 µL

Performance 
Sensitivity

< 1 amol ATP

< 1 zmol Firefly luciferase

Dynamic Range> 6 orders of magnitude
Injectors

accuracy better 98 % over entire volume range

precision better 98 % over entire volume range

Miscellaneous 
Dimensions480 x 570 x 380 mm (w x d x h)
RegulationsCE, UL

 

Accessories

LBIS immunoassay software

The Windows software for instrument operation and data evaluation supports competitive (LIA) and immunometric (ILMA) assays with superior curve fitting algorithms increasing the reliability of the calculated results especially at the lower concentrations.

It enables worklist download and sample data upload to host computers.

Up to 4 instruments connceted to a single computer can be run with one LBIS software.

Applications

ATP Monitoring

ATP determination in lung epithelia cells: Seminario-Vidal et al. (2009). Thrombin Promotes Release of ATP from Lung Epithelial Cells through Coordinated Activation of Rho- and Ca2+-dependent Signaling Pathways; JBC 284 (31), 20638-20648 Read more

Cell Viability

ATP production in neuroblastoma cells: Ehrich et al. (2011). Fullerene antioxidants decrease organophosphate-induced acetylcholinesterase inhibition in vitro. Toxicology in Vitro 25 (1), 301–307 Read more

Cellular Chemiluminescence

Cellular luminescence in male SHR: Iliescu et al. (2007). Impact of androgen-induced oxidative stress on hypertension in male SHR. AJP Regulatory, Integrative and Comparative Physiology 292 Read more

Reactive Oxygen Species (ROS)

ROS measurement in Medicago truncatula: Wang et al. (2011). Ontogeny of embryogenic callus in Medicago truncatula: the fate of the pluripotent and totipotent stem cells. Analls of Botany 107, 599-609 Read more

FcR crosslinking–induced generation of reactive oxygen species: Tsuboi et al. (2011). Regulation of human neutrophil Fcγ receptor IIa by C5a receptor promotes inflamma-tory arthritis in mice. Arthritis & Rheumatism 63 (2), 467–478 Read more

Luminescent Immunoassays

Chemiluminescent Immunoassay: Bidlingmaier et al. (2009). High-Sensitivity Chemiluminescence Immunoassays for Detection of Growth Hormone Doping. Sports Clinical Chemistry 55 (3), 445-453 Read more

Brenner et al. (2011). I-131 Dose Response for Incident Thyroid Cancers in Ukraine Related to the Chornobyl Accident. Environmental Health Perspect 119 (7), 933–939 Read more

Reporter Gene Assays (incl. DLR)

Luciferase Assay: Moncorgé et al.(2010). Evidence for avian and human host cell factors that affect the activity of influenza polymerase. Journal of Virology 84 (19), 9978-9986  Read more

Dual-Luciferase Assay in rabbit amnion: Jeng et al(2009). Characterization of the Cyclic Adenosine Monophosphate Target Site in the Oxytocin Receptor Gene in Rabbit Amnion. Biology of Reproduction 81 (3), 473-479  Read more

Calcium Monitoring

Calcium determination with Aequorin in Arabidopsis cells: Harada et al. (2009). Measurement of Changes in Cytosolic Ca2+ in Arabidopsis Guard Cells and Mesophyll Cells in Response to Blue Light; Plant and Cell Physiology 50 (2), 360-373 Read more
 

Further applications

DNA probe assays - Environmental monitoring (Vibrio fischeri test) - Enzyme activities

Download
Brochure
Format Size
AutoLumat LB 953 Brochure 2009 de, en PDF 1.01 MB
Bioanalytic Instruments - Product Portfolio Brief overview scope of supply Bioanalytic (June 2014) de, en PDF 693.31 KB
Order Information Consumables & QC Test Devices Version Jan. 2017 de, en PDF 153.63 KB
Press & Media
Format Size
2014-02 (February 2014) AutoLumat LB 953 Application Information AutoLumat LB 953 - Berthold Technologies Newsletter February 2014 de, en PDF 140.48 KB

News about this product

Oxidative stress has been implicated in the etiology of male infertility.

Read more
16.10.2013