TriStar² LB 942 Multidetection Microplate Reader

The Even-More-Value-for-Money Multimode Reader

With TriStar² the multi-technology microplate reader platform has developed into its second generation setting new standards in modularity, performance and user-friendliness.

  • Luminescence
  • BRET and BRET²
  • Fluorescence
  • FRET
  • Absorbance

The TriStar² has been developed to support a fully modular approach. Any combination of reading technologies and options can be composed maintaining all possibilities of later upgrades.

The optical system ONE-4-ALL with a new revolutionary design is the first time a single optical system has been employed in a multi-technology reader that matches the performance of individual dedicated optical devices.

Tabs

Features

Built-in Reagent Injectors

Up to 3 reagent injectors can be set up to dispense multiple activators or detection reagents at any time and with user-adjustable volumes during the measurement.
Berthold Technologies´ injectors are based on the proprietary JET Injection technology. Superior accuracy and precision is combined with excellent mixing performance. Variable injections speeds and "friendly" material enable even injection of live cells (e.g. in Aequorin based GPCR screening).
One of the injector tips is located in reading position enabling the measurement of very fast labels, e.g. Acridinium esters, and to inject activators into a running kinetic.
The ergonomic design of the housing provides front access of the injectors keeping the reagent reservoirs within reach and within eyesight. A through allows keeping the reagent bottles on ice or elevated temperatures – just as required.

Wash Plate

Excess fluids from priming and washing procedures are safely discarded via a specially designed wash plate.

Temperature Control

A temperature controlled microplate compartment ensures stable conditions whenever temperature sensitive enzymes or cells are in use.

Front Access of Reagents

The reagent reservoirs can be placed on the front for easy access and monitoring. A smoked see through lid protects the reagents from light. A base trough allows for the use of ice to keep the reagents cool.

Modularity

In the TriStar² any of reading technologies can be combined with each other and any of the options added.

Specifications
Detection Deviceslow-noise photomultiplier tube operated in single photon counting mode, spectral range 340 to 650 nm
photo diode, spectral range 200 – 1000 nm
Excitation Sourceshalogen lamp
Sample/Plate Formats6 – 384 well plates
Terasaki plates
Petri dishes
filter membranes
Temperature Controlup to 42 °C
including Peltier cooling of photomultiplier
Shaking3 modes, 3 velocities
Scanningup to 10,000 points per well
Injectionup to 3 JET injectors
variable volume 10 – 100 µL
variable speed
Kinetics Operationminimum resolution 0.05 sec
maximum duration up to 7 days
Performance 
Luminescence   <6 amol ATP per well
<3 zmol Firefly luciferase per well (equals less than 800 molecules)
Crosstalk   5 x 10-6
Fluorescence   <0.3 fmol FITC per well
Absorbanceaccuracy <2 % deviation (@ 2 OD, 405 nm)
precision <0.6 % CV (@ 2 OD, 405 nm)
Dynamic range>6 decades (PMT)
4 decades (photo diode)
Injectorsaccuracy better 98 % over entire volume range
precision better 98 % over entire volume range
Miscellaneous 
Dimensions   390 x 470 x 345 mm (w x d x h)
incl. injectors
OSWin XP, Win Vista, Win 7
Regulations   CE

 

Accessories

ICE software

The TriStar² comes standard with the Research version of the ICE (Instrument Control and Evaluation) software. The ICE Research version supports ratio calculation for reporter gene applications, ADP and ATP determinations and kinetics functionality enzyme activities. Point-to-point curve fitting enables simple work with standard curves.
The ease of use during protocol creation, measurement and data export has been achieved with the wizard-driven and clearly structured software.
Advanced functionality can be added to the ICE software by upgrading to the Advanced version, e.g.

  •      extended reports for standard curve assays
  •      additional curve fit algorithms: linear regression, spline
  •      use of master and reference curves
  •      cut-off assay data reduction

Mikrowin software

This optional software combines operation and definition of instrument settings as well as data reduction and evaluation. The wells for measurement and injection can be selected independently. Various operations can be picked and placed in any order to accommodate for all different requirements of the assays to be performed.
Kinetic data reduction and graphical display of the respective curves help the user to judge the results.
Any type of ratio calculation, e.g. DLR®, or other mathematical formulas can be linked to every well individually.
Standard curve fitting and calculation of unknown samples is available for those users looking for quantification of their results (Advanced I and II).
All data can be exported in EXCEL® or ASCII formats. Instrument settings, user
signatures and file information are stored with the results.
The password system and the audit trail function of the Advanced I and II versions are important tools for data security.

Adapters

For non-microplate shaped sample containers, e.g. Petri dishes, Terasaki plates and even filter membranes, special adapters are available to enable reading of this kind of lab ware in the Mithras.

Selected filter sets

For specific applications such as BRET, BRET² and ChromaGlo Berthold Technologies´ application specialists together with scientific users and reagent manufacturer´s scientists have put together optimised filters matching the reagents requirements and ensuring best performance.

BRET package4 filters for BRET and BRET²39350
BRET high efficiency2 filters for BRET with improved transmission53431
BRET² high efficiency2 filters for BRET² with improved transmission53432
ChromaGlo™2 filters specific for green and red emitting luciferases43544

 

Excitation and Emission Filters

More than 100 high quality filters for absorbance, fluorescence excitation and emission as well as luminescence are available. For details see optical filters.

Test Plates & QC Services

For continuous verification of the instrument performance test plates or test kits can be applied by the user and Performance Qualification services done by experienced Berthold Technologies personnel are available.
 

Cleanit Daily Injector Cleaning Solution

For proper maintenance of the injection system the Cleanit Daily solution is recommended for regular cleaning. Daily cleaning ensures that accuracy and precision as well as a long life time of the injectors will be maintained.

Applications

Reporter gene assays

Bacterial Luciferase assay in Ph. phosphoreum: Kamnev et al.(2013). Effects of americium-241 and humic substances on Photobacterium phosphoreum:
Bioluminescence and diffuse reflectance FTIR spectroscopic studies. Spectrochimica Acta Part A 100 (2013), 171–175 Read More

eGFP/dsRed Reporter assay in N. benthamiana leaves: Stauffer et al. (2010). Polypyrimidine tract-binding protein homologues from Arabidopsis underlie regulatory circuits based on alternative splicing and downstream control. The Plant Journal 64, 243–255 Read more

Dual Luciferase Reporter Assay: Juraver-Geslin et al. (2011). Barhl2 limits growth of the diencephalic primordium through Caspase3 inhibition of β-catenin activation. PNAS 108 (6), 2288-2293 Read more

Protease assays

Protease assay with fluorogenic peptide substrate: Jo et al. (2012). Development of α-Helical Calpain Probes by Mimicking a Natural Protein-Protein Interaction. J. Am. Chem. Soc, 2012 Oct 24;134(42) Read More

Immunoassays

ELISA: Vogt et al. (2011). Poorly Neutralizing Cross-Reactive Antibodies against the Fusion Loop of West Nile Virus Envelope Protein Protect In Vivo via Fcγ Receptor and Complement-Dependent Effector Mechanisms. JOURNAL OF VIROLOGY, 11567–11580 Read more

Caspase assays

Caspase assay in clams: Strahl et al. (2010). Cell turnover in tissues of the long-lived ocean quahog Arctica islandica and the short-lived scallop Aequipecten opercularis. Marine Biology 157 (6), 1283-1292 Read more

Cytotoxicity

LDH release (Cytotox Assay) Hamon et al. (2011). K+ Efflux Is Required for Histone H3 Dephosphorylation by Listeria monocytogenes Listeriolysin O and Other Pore-Forming Toxins. Infection and Immunity 79 (7), 2839-2846 Read more

BRET and BRET²

GPCR heterodimerization: Bai et al. (2014). Heterodimerization of apelin receptor and neurotensin receptor 1 induces phosphorylation of ERK1/2 and cell proliferation via Gαq-mediated mechanism. Journal of Cellular and Molecular Medicine 18: 2071–2081. doi: 10.1111/jcmm.12404 Read more

GPCR oligomerization: Wade et al. (2011). Relationship between Homo-oligomerization of a Mammalian Olfactory Receptor and Its Activation State Demonstrated by Bioluminescence Resonance Energy Transfer. The Journal of Biological Chemistry 286, 15252-15259 Read more

Cell proliferation

ATPLite assay: Stavru et al. (2011). Cell Listeria monocytogenes transiently alters mitochondrial dynamics during infection. PNAS 108 (9), 3612-3617 Read more

Cell migration

Transwell permeability assay: Sume et al. (2010). Epithelial to Mesenchymal Transition in Gingival Overgrowth. The American Journal of Pathology 177 (1), 208–218 Read more

Cellular luminescence / Phagocytosis

Cellular chemiluminescence: Wan et al. (2010). A high throughput chemiluminescence method based on molecularly imprinted sol-gel films for determination of sibutramine. Advanced Material Letters 1 (2), 164-169 Read more

Enzyme kinetics

Proteasome assay: Jo et al. (2010). Natural Product Celastrol Destabilizes Tubulin Heterodimer and Facilitates Mitotic Cell Death Triggered by Microtubule-Targeting Anti-Cancer Drugs. PLoS ONE 5 (4), e10318 Read more

Reactive Oxygen Species (ROS)

ROS production in A549 cells: Zhang et al. (2008). Interactions between PBEF and oxidative stress proteins-a potential new mechanism underlying PBEF in the pathogenesis of acute lung injury. FEBS Letters 582 (13), 1802–1808 Read more

Permeability/Migration

Transwell assay of fluorescent microsphere: Gali et al. (2010). In Vitro Evaluation of Viability, Integrity, and Inflammation in Genital Epithelia upon Exposure to Pharmaceutical Excipients and Candidate Microbicides. Antimicrobial Agents and Chemotherapy 54(12), 5105–5114 Read more


Further Applications

ATP determination - Apoptosis - Calcium monitoring - Chemotaxis - Cytokine analysis - ChromaGlo™ assays - DNA quantification - FRET - GPCRs / 7TM receptors - Ion channel studies - Kinase assays - Membrane potential - Nucleic acid quantification - Protein concentration - Protein-protein interactions - Receptor binding studies - SNP determination

Download
Application Note
Format Size
Screening of GPCR-anatgonists using the Ca Mobilisation Assay Application Note describing usage of the Calcium Mobilisation Assay for GPCR-antagonist screening. de, en PDF 1009.46 KB
Brochure
Format Size
Bioanalytic Instruments - Product Portfolio Brief overview scope of supply Bioanalytic (June 2014) de, en PDF 693.31 KB
Filter Data Sheet Dec 2013-V3 de, en PDF 604.67 KB
LB 942 TriStar² & TriStar²S Broschure en PDF 4.58 MB
Order Information Consumables & QC Test Devices Version Jan. 2017 de, en PDF 153.63 KB
Press & Media
Format Size
2012-02 (February 2012) TriStar2 microplate reader NL TriStar2 issue Berthold Technologies Newsletter February 2012 de, en PDF 112.41 KB
2012-07 (July 2012) New website & TriStar2 microplate reader NL TriStar2 issue Berthold Technologies Newsletter July 2012 de, en PDF 180.9 KB
2013-01 (January 2013) Photon Counting & TriStar² NL The Truth about PMTs & TriStar² Issue - Berthold Technologies Newsletter Jan. 2013 de, en PDF 130.2 KB
Invitation 8th BioDetectors Conference Torino, Italy, 25th/26th September 2014 en PDF 298.47 KB
Press Release TriStar² Multimode Microplate Reader April 2014 en DOCX 2.2 MB
Stampa Tristar2&NightSHADE_May2012 italiano en PDF 72.71 KB
TriStar2S_graphics_fullpage_ad graphics for full page print advertisement (A4, letter and midadlantic); Sept. 2014 en ZIP 24.85 MB

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