bScreen Label-Free Microarray System LB 991

The bScreen LB 991 Label-Free Microarray System with Biametrics SCORE technology (Single Colour Reflectometry) enhances Biolayer Interference technology (BLI). It combines the advantages of label-free detection with the throughput and flexibility of microarray technologies. Experience the robust, user-friendly and affordable workflow that minimizes hands-on time while increasing information content. The Next Generation Microarray SCORE technology provides access to abroad range of sample types (small molecuels, fragmensts, proteisn, peptides, viruses, whole cells, nanoparticles...) in complex matrices (buffer, DMSO, serum, whole blood, milk...). It allows for comprehensive kinetic and thermodynamic evaluation of biomolecular binding while avoiding the typical pitfalls of label-based technologies. The bScreen LB 991 sets a new standard in high-throughput label-free screening with unprecedented performance or quality.

Tabs

Features

SCORE Technology

The proprietary SCORE technology (Single Colour Reflectometry) utilises the interference of monochromatic light, which is reflected at the interface between the biolayer and the sensor chip (see Figure 1). The intensity of the reflected light depends on the physical thickness and refractive index of the biolayer, which changes upon binding of molecules. The SCORE technology transfers this principle onto a robust Microarray format, enabling the simultaneous binding kinetic analysis of thousands of interactions between an immobilised and a soluble partner in real-time and label-free.

 

 

 

 

Information-rich Data

Get all binding parameters for up for up to 22,500 candidates in a single run:

  • Full kinetic information (KD, ka, kd)
  • Concentration (detection limit < 1 pg/mm2)
  • Thermodynamic data (ΔH, ΔS) with optional temperature control

Scales to your Reseach

The bScreen LB 991 combines the worlds of kinetic analysis and high throughput microarray screening. The customisable array format enables outstanding flexibility: Generate kinetic binding curves from a single spot to high density arrays with up to 22,500 interaction sites per run and produce more than 110,000 kinetic data sets in an 8 hours work shift.

Economic Workflow

Using high density (biomolecular) interaction arrays, the cost and time per analysed interaction can be reduced dramatically. With its optimised flow channel and precision fluidics the consumption of precious sample material can be minimised. The intuitive software package guarantees easy and streamlined operation to let you stay focused on your research.

Unmatched Content and Quality

The microarray based SCORE technology enables parallelised testing of on chip replicates under identical conditions for best reliability and superior data quality. Immobilised binding partners in different concentrations facilitate the in-run assay optimisation without any time consuming pre-runs.

Accomplish more, faster

Perform your library screening and kinetic lead characterisation in parallel. The label-free SCORE technology avoids unspecific interaction of the label with the surface and eliminates the risk of label interference with the biomolecular interaction.

 

 

 

 

 

 

 

 

Specifications

 

Performance Characteristics

Sample TypesLOW MW drug candidates (MW < 150 Da) up to high MW proteins, DNA, RNA, polysaccharides, lipids, cells, viruses, nanoparticels in various matrices (DMSO-containing buffers, urin
Sample Format

Array chips in standard microscopy slide format with specialized biopolymer coating

Compatible to most µArray spotters

Typical Throughput

Up to 240,000 interactions/24 h (Direct binding assay up to 10,000 targets per assay, assay time 1 h),

Antibody screening: 240,000 samples / 24 h

Typical Evaluation TimeScreening: < 1 h; Kinetics: 1 to 2 h
Measurement Parameters/InformationKinetic and affinity data (KD, ka, kd), specifity, concentration, thermodynamic parameters (ΔH, ΔS)
Detection Limits< 1pg/mm2
Rate Constants

ka: 103 - 107 M-1s-1 (higher for macromolacular analytes)

kd: 10-6- 0.5 s-1

 

 

Technical Specifications

Detection TechnologyBiametrics Single Color Reflectometry (SCORE)
Sample Consumption50 - 500 µl (application dependent)
Inline Reference SubstractionAutomatic (Reference Spots)
AutomationAutomated measurement cycle with up to 5 samples/reagents
InterfacingImport and export function and connection to LIMS-systems
Software

Wizard guidance through complete measurement process from protocol generation to data evaluation with graphical user interface.

OS: Win XP, Win Vista, Win 7, Win 8, Win 8.1, Win 10

Dimensions

W: 66 cm, D: 61 cm, H: 33 cm

Weight: 35 kg

 

Accessories

Consumables

 

The bScreen ist accepts biofunctionalized arrays-chips in standard 75 x 25 mm2 micorscopy slide format as consumables. The chips are compatible to commercial µArray spotters.

 

Array-chips with biofunctionalization
63114 BM array-chip, 2D biopolymer coating, carboxy functionalization
63115 BM array-chip, 3D biopolymer coating, carboxy functionalization
63116 BM array-chip, 2D biopolymer coating, amino functionalization
63117 BM array-chip, 3D biopolymer coating, amino functionalization
63118 BM array-chip, 2D biopolymer coating, NHS-ester functionalization
63119 BM array-chip, 3D biopolymer coating, NHS-ester functionalization
63120 BM array-chip, 2D biopolymer coating, streptavidin functionalization
63121 BM array-chip, 3D biopolymer coating, streptavidin functionalization
  Custom specific coatings

 

Applications

One Platform, multiple applications

Advance your research with the label-free b-screen system. The flexibility of the b-screen system enables fast assay development for biomolecular interaction studies. Its robust technology enables analysis of a wide range of matrices ranging from buffer, serum, whole-blood, salvia, cell culture media to crude lysates.

Protein/Protein Interactions

Meet your throughput needs

  • Epitope-mapping
  • Kinetic characterisation
  • Proteome analysis
  • Post-translational analysis
 

Pharmaceutical Screening

Search for biological active compounds

  • Kinetic characterisation of receptor ligand interactions
  • Characterisation and optimisation of therapeutic antibodies
  • Inline process-control for biologics
  • Lead optimisation
 

Cell based Assays

Monitor whole cells

  • Observe binding even on the cell surface
  • Measure in continuous flow
  • Glass type array chip with cell binding biopolymers
  • Screening of cell arrays
 

Diagnostic Research

Fast development of multi parameter assays

  • Various sample materials (blood, salvia, …)
  • Flexible assay types (direct, sandwich, binding-inhibition, competitive and replacement assays)
  • Quantitative
  • No or reduced sample pre-treatment

 

 
 
     
Download
Application Report
Format Size
High Throughput Peptide Screening for Next Generation Anti-Tumour Agents Read how the bScreen LB 991 label-free reader revolutionises pharmaceutical screening applications. de, en PDF 1.39 MB
Label-free Screening of Human Automimmune Diseases en PDF 373.53 KB
Application Note
Format Size
Next Generation Microarrays Live Tour 2016 de, en PDF 250.51 KB
Brochure
Format Size
bScreen LB 991 Brochure en PDF 3.32 MB
Press & Media
Format Size
Picture of bScreen LB 991 High resolution picture en JPG 4.85 MB
Press Release bScreen LB 991 January 2015 en DOCX 440.33 KB
Press Release Cooperation Berthold and Biametrics February 2015 en DOCX 79.2 KB

News about this product

Berthold Technologies und Biametrics laden Sie ein zur Teilnahme an der Next Generation Microarrays Live Tour 2016.

Read more
30.08.2016
Berthold Technologies and Biametrics are inviting you to participate in and enjoy the Next Generation Microarrays Tour 2016. Read more
30.08.2016
co-operation between Berthold Technologies and its new technology partner Biametrics GmbH, a spin-off of Tübingen University, for novel novel solutions in label-free analysis of bio-interactions Read more
16.02.2015