Multimode Mikroplatten Reader im Vergleich
| Tristar 3 | Tristar 5 | |
|---|---|---|
| Optical system | Single path for all detection technologies | Single path for all detection technologies |
| Light source |
|
|
| Spectral range Absorbance | 200-1000 nm | 200-1000 nm |
| Spectral range Luminescence | Standard: 280-650 nm Extended: 280-850 nm | Standard: 280-650 nm Extended: 280-850 nm |
| Spectral range Fluorescence | Excitation: 230-1000 nm Emission: Standard 280-650 nm, Extended 280-850 nm | Excitation: 230-1000 nm Emission: Standard 280-650 nm, Extended 280-850 nm |
| Bottom reading | ||
| Filters | ||
| Monochromator | ||
| Filter identification | Manual | Manual |
| Wavelength scanning | ✘ |
|
| Monochromator stray light | 10-6 | |
| Monochromator bandwidth | 4-22 nm |
| Tristar 3 | Tristar 5 | |
|---|---|---|
| Injectors | Up to 3 JET injectors with frictionless operation | Up to 3 JET injectors with frictionless operation |
| Injector volume | 10-100 µL in 1 µL steps | 10-100 µL in 1 µL steps |
| Injector accuracy | Better than 2% across the entire volume range | Better than 2% across the entire volume range |
| Injector precision | Better than 2% across the entire volume range | Better than 2% across the entire volume range |
| Tristar 3 | Tristar 5 | |
|---|---|---|
| Microplate formats | 6- to 384-well | 6- to 384-well |
| Plate height adjustment | 3 different frames depending on plate type | 3 different frames depending on plate type |
| Additional sample formats (optional) |
|
|
| Optional Microplate Stacker |
| Tristar 3 | Tristar 5 | |
|---|---|---|
| Absorbance precision | Better than 0.6% | Better than 0.6% |
| Absorbance accuracy | Better than 2% | Better than 2% |
| Luminescence sensitivity | <6 amol (0.03 pM) ATP/well (96-well plate) | <6 amol (0.03 pM) ATP/well (96-well plate) |
| Fluorescence sensitivity | <7 amol (0.35 pM) Fluorescein (384sv plate) | <7 amol (0.35 pM) Fluorescein (384sv plate) |
| TRF sensitivity | - | 5 amol Eu (96-well plate) |
| Crosstalk | 10-6 | 10-6 |
| Dynamic range | >6 orders of magnitude 0-3.5 OD | >6 orders of magnitude 0-3.5 OD |
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